Introduction:Radiotherapy is a standard and effective modality for breast cancer treatment, through induction of DNA damages notably DNA double-strand breaks which are involved in radiation-induced cell death. All radiation-induced DNA damages are subjected to various repair processes, therefore, interference in the DNA repair pathways might result in radio-resistance. Non-coding RNAs are a diverse group of functional RNA molecules that are not translated into proteins. Recent studies have shown that radiation can cause expression changes in noncoding RNAs. Materials and Methods:MCF-7 and MDA-MB-231 cell lines were grown in a DMEM culture medium supplemented with fetal bovine serum and antibiotics. At exponential growth, cells were exposed to various doses of megavoltage X-rays. 24 and 48 h after irradiation cells were harvested, RNA was extracted and cDNA was synthesized. The expression level of lncRNAs was measured using quantitative real-time PCR. Results:This study showed that radiation could increase DANCRand TUG1lncRNAs expression in breast cancer cell lines 24 and 48 h after receiving radiation. Also, the results suggested that after radiation, the expression of DANCRin the radioresistant cell line was higher than the radiosensitive one; in the case of TUG1, it’s unlike DANCR. Conclusions:The radiation increased the expression of DANCR and TUG1 lncRNAs in breast cancer cell lines because the expression of DANCRin the MDA-MB- 231 was higher than in the MCF-7. In contrast, the expression level of TUG1 in MCF-7 was higher than the MDA-MB-231. Therefore, lncRNAs, DNACR, and TUG1might play a role in the radioresistance and radiosensitivity of breast cancer, respectively.
Nejadtaghi, M. & Mozdarani, H. (2023). The Effect of Radiation on the Expression Level of DANCR and TUG1 LncRNAs in Breast Cancer Cell Lines. Journal of Applied Biotechnology Reports, 10(2), 1009–1017. https://doi.org/10.30491/jabr.2023.366752.1570
MLA
Nejadtaghi, M., & Mozdarani, H. "The Effect of Radiation on the Expression Level of DANCR and TUG1 LncRNAs in Breast Cancer Cell Lines", Journal of Applied Biotechnology Reports, 10, 2, 2023, 1009–1017. doi: 10.30491/jabr.2023.366752.1570
HARVARD
Nejadtaghi, M., & Mozdarani, H. (2023). 'The Effect of Radiation on the Expression Level of DANCR and TUG1 LncRNAs in Breast Cancer Cell Lines', Journal of Applied Biotechnology Reports, 10(2), pp. 1009–1017. doi: 10.30491/jabr.2023.366752.1570
CHICAGO
Nejadtaghi, M. & Mozdarani, H., "The Effect of Radiation on the Expression Level of DANCR and TUG1 LncRNAs in Breast Cancer Cell Lines." Journal of Applied Biotechnology Reports, 10 2 (2023): 1009–1017, doi: 10.30491/jabr.2023.366752.1570
VANCOUVER
Nejadtaghi M., Mozdarani H. The Effect of Radiation on the Expression Level of DANCR and TUG1 LncRNAs in Breast Cancer Cell Lines. J Apple Biotechnol Rep. 2023;10(2):1009–1017. doi: 10.30491/jabr.2023.366752.1570
