DNA Electrochemical Nanobiosensors for the Detection of Biological Agents

Document Type : Review Article


1 Department of Biology, Imam Hossein University, Tehran, Iran

2 Department of Chemistry, Imam Hossein University, Tehran, Iran


In this study, we explained a nanobiosensor for DNA sequence detection, featuring sequence specificity, cost efficiency, speed, and ease of use. Without the need for labels or indicators, it may be ideal for the detection of biological agents. This review describes recent advances in electrochemical impedance spectroscopy (EIS) with an emphasis on using nanoparticles and nanotechnology. A powerful biosensor system requires a high-performance biosensor component as well l as a user-friendly instrumental setup. However, biosensor setups have to be adapted to specific applications. Rapid, selective and sensitive detection technologies for biological agents are critical in clinical diagnosis, environmental monitoring and food safety. Recent developments in nanomaterial create many opportunities to advance DNA sensing and gene detection. The fact that gold nanoparticles are able to provide a stable immobilization of biomolecules that retain their bioactivity is a major advantage for the preparation of biosensors. Although, there are a lot of researches reporting electrode modification by different nanomaterial to improve the DNA biosensor performance, the preparation of nanomaterial or the electrode modification strategy is often relatively complex. Furthermore, some DNA biosensors based on nanomaterial modification are still very limited for the improvement of DNA biosensor performance. Thus, the construction of nanostructure modified electrode by a simple strategy to improve the DNA detection sensitivity is highly desirable.