Journal of Applied Biotechnology Reports

Journal of Applied Biotechnology Reports

Fabrication of an Amperometric Glucose Biosensor Based on a Prussian Blue/Carbon Nanotube/Ionic Liquid Modified Glassy Carbon Electrode

Document Type : Original Article

Authors
Sharareh Sajjadi 1
Amir Homayoun Keihan 2
Parviz Norouzi 3
Mohammad Mahdi Habibi 3
Khadijeh Eskandari 4
Najmeh Hadizadeh Shirazi 1
1 Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, Iran
2 Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
3 Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran
4 Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Abstract
An amperometric glucose biosensor was developed based on synergistic contributions of prussian blue (PB) and a bucky gel (BG) consisting of carbon nanotubes (CNTs) and ionic liquid (IL). The PB nanoparticles were first deposited onto the surface of a BG modified glassy carbon (GC) electrode (BG/GC). Then, the Ni2+ ions were electrochemically inserted into the PB lattice to improve its stability at physiological pH. Afterwards, Glucose oxidase (GOx) was immobilized on the BG/GC electrode using a cross-linking method. Amperometric measurements of glucose were performed at −0.05 V vs. Ag/AgCl in 0.05 M phosphate buffer solution at pH 7.4. The glucose biosensor exhibited a sensitivity of 45.03 µA/(mM.cm2) with a detection limit of 5×10-7 M. The amperometric response was linear in the range of 5×10-7 to 8.3×10−4 M.
Keywords
Glucose
Biosensore
Nanoparticle
Carbon Nanotube
Ionic Liquid
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Journal of Applied Biotechnology Reports
Volume 4, Issue 2
Spring 2017
Pages 603-608

  • Receive Date 11 April 2017
  • Revise Date 11 May 2017
  • Accept Date 11 July 2017