Journal of Applied Biotechnology Reports

Journal of Applied Biotechnology Reports

Novel PCR-ELISA Technique as a Good Substitute in Molecular Assay

Document Type : Review Article

Authors
1 Department of Biology, Damghan Azad University, Damghan, Iran
2 Department of Biology, Faculty of Science, Imam Hossein University, Tehran, Iran
3 Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
Abstract
Due to the spread of infectious diseases, the existence of a rapid and sensitive detection method is necessary today. Polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) is a simple manner for detection of microorganism. For example, bacteria, viruses, fungi and others based on nucleic acid sequence. A large number of samples can be screened by this technique simultaneously, so it is not time consuming and is a quick manner. The high sensitivity and specificity of PCR-ELISA make it a powerful technique by simple laboratory facilities. As a result it can be an excellent substituted manner for analysis and detectionin different various fields.
Keywords

Volume 4, Issue 2
Spring 2017
Pages 567-672

  • Receive Date 11 June 2017
  • Accept Date 11 July 2017