Journal of Applied Biotechnology Reports

Journal of Applied Biotechnology Reports

Rapid Detection of Vibrio Cholerae by Polymerase Chain Reaction based on Nanotechnology Method

Document Type : Original Article

Authors
Gholam Reza Herfehdoost 1
Mahdi Kamali 2
Hamid Reza Javadi 2
Davood Zolfagary 2
Asghar Emamgoli 2
Ali Choopani 3, 4
Bahareh Ghasemi 4
Saeideh Hossaini 4
1 Neuroscience Research Center, Baqiyatallah university of Medical Sciences, Tehran, Iran
2 Nanobiotecnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
3 Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
4 Department of Biology, Payamnoor University, Tehran, Iran
Abstract
Quick identification of Vibrio Cholerae in epidemics is important, on the other hand; conventional methods are time-consuming and costly. The aim of this study was to develop a rapid, inexpensive and high sensitivity method for quick identification of Vibrio Cholerae. For this purpose we designed a PCR detection based on magnetic nanoparticles for identification of bacterial DNA by PCR Dynabead. So we used the biotinylated Probe for binding to DNA extracted from Vibrio Choleraeand other bacterial species (Salmonella, Shigella, Pseudomonas, E.coli) Using magnetic bead isolated with magnetic field, the Vibrio genome-specific primers (HlyA) for pathogen detection (PCR) was used. The results showed specific band was just for Vibrio Cholera (PCR positive); therefore designed probe was specific for Vibrio Cholerae. According to the findings, this study is characterized the high sensitivity of PCR using biotin-containing probes for DNA of Vibrio Cholera in contrast to the traditional methods.
Keywords
Magnetic nanoparticles
Detection
Vibrio Cholerae
PCR
Biotinylated Probe
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Journal of Applied Biotechnology Reports
Volume 1, Issue 2
Spring 2014
Pages 59-62

  • Receive Date 28 March 2014
  • Revise Date 03 May 2014
  • Accept Date 03 May 2014