Optimization of Extracellular Lipases Production by Fungal Strains Isolated from the Soil Enriched with the Oil of Pistacia lentiscus

Ouartsi, Nouha; Ghania, Bourzama; Benouagueni, Sarah; Benhassine, Yousra; Ouled-Haddar, Houria; Sahli, Sara; Hamaidi, Lamia

doi:10.30491/jabr.2024.477960.1778

Optimization of Extracellular Lipases Production by Fungal Strains Isolated from the Soil Enriched with the Oil of Pistacia lentiscus

Document Type : Original Article

Authors

Nouha Ouartsi ¹

Bourzama Ghania ¹

Sarah Benouagueni ¹^{, 2}

Yousra Benhassine ³

Houria Ouled-Haddar ⁴

Sara Sahli ⁵

Lamia Hamaidi ⁵

¹ Laboratory of Microbiology and Molecular Biology, Badji Mokhtar Annaba-University. 12, P.O. Box, 23000 Annaba, Algeria

² Laboratory of Biochemistry and Applied Microbiology, Badji Mokhtar Annaba-University. 12, P.O. Box, 23000 Annaba, Algeria

³ Laboratory of Biochemistry and Environmental Toxicology, Badji Mokhtar Annaba-University. 12, P.O. Box, 23000 Annaba, Algeria

⁴ Laboratory of Molecular Toxicology, University of Jijel, Jijel, Ouled Aïssa 18000,, Algeria

⁵ Biochemistry Department, Badji Mokhtar Annaba-University. 12, P.O. Box, 23000 Annaba, Algeria

10.30491/jabr.2024.477960.1778

Abstract

Introduction: Lipases are enzymes that catalyze a large number of hydrolysis reactions leading to a vast diversity of industrial applications. Microbial lipases constitute an important group of biotechnologically valuable enzymes, mainly because of the versatility of their properties and ease of their production and extraction. The aim of this research work is the isolation of lipolytic fungal strains from the soil enriched with the oil of Pistacia lentiscus in the Annaba region of Algeria.
Materials and Methods: Fungi isolation was carried out on Potato Extract Agar (PDA) at 28 °C. Lipase activity was determined through the estimation of free fatty acids by titrimetry, optical density and final pH. The optimization of lipase production was achieved for several factors, namely, agitation, pH, temperature, biomass, carbon source, lipidic substrate, and time.
Results: Three strains were isolated and identified as Aspergillus sp., Fusarium sp., and Penicillium sp., with the latter exhibiting the highest lipolytic activity. The optimization indicated that these strains were able to hydrolyse different fatty substances with high activity under different conditions.
Conclusions: The obtained results show that pistachio oil was considered a novel inducer of the lipase-producing fungal strains.

Keywords

East Algeria

Molds

Lipase

Pistachio Oil

Production Conditions