Analysis of the Chemical Composition and Evaluation of the Antioxidant, Anti-inflammatory and Hemolytic Activities of the Essential Oil and its Oxygenated Fraction of Chrysanthemum coronarium

Bendiabdellah, Amel; Mami, Imane Riheb; DIB, Mohammed El Amine; Djabou, Nassim; Muselli, Alain

doi:10.30491/jabr.2024.437240.1699

Analysis of the Chemical Composition and Evaluation of the Antioxidant, Anti-inflammatory and Hemolytic Activities of the Essential Oil and its Oxygenated Fraction of Chrysanthemum coronarium

Document Type : Original Article

Authors

Amel Bendiabdellah ¹

Imane Riheb Mami ¹

Mohammed El Amine DIB ¹

Nassim Djabou ²

Alain Muselli ³

¹ Laboratory of Natural and Bioactive Substances (LASNABIO), University of Tlemcen, Tlemcen, Algeria

² Laboratory of Organic Chemistry, Natural Substances and Analysis (COSNA), University Aboubekr Belkaïd, Tlemcen, Algeria

³ Laboratoire Chimie des Produits Naturels, Universite de Corse, UMR CNRS 6134, Corté, France

10.30491/jabr.2024.437240.1699

Abstract

Introduction: The main objective of this research was to study the chemical composition, as well as the antioxidant, anti-inflammatory, and hemolytic activities of the aerial part of the essential oil of Chrysanthemum coronarium and its isolated oxygenated fraction.
Materials and Methods: The oxygenated fraction was isolated from the oil by column chromatography. Analyses by GC coupled with mass spectrometry allowed for the characterization of these components. The antioxidant properties of the essential oil and its oxygenated fraction were evaluated using the DPPH and FRAP methods. The anti-inflammatory effect was characterized by the denaturation of proteins. In addition, the hemolytic impact was studied through a suspension of erythrocytes in human blood.
Results: The essential oil studied was characterized by a composition dominated by oxygenated monoterpenes (39.3%), followed by hydrocarbon sesquiterpenes (21.1%) and oxygenated sesquiterpenes (16.5%). The oxygenated fraction consisted mainly of perillyl alcohol (14.3%) and lyratyl acetate (14.5%). The results of the DPPH and FRAP tests showed significant antioxidant activity of the essential oil (IC50 = 3 ml/L) and the oxygenated fraction (IC50 = 4.4 ml/L), surpassing that of the synthetic antioxidant BHT. Additionally, the oxygenated fraction exhibited perfect anti-inflammatory activity, compared to essential oil and diclofenac sodium. The toxicity assessment on human erythrocytes shows that both essential oil and oxygenated fraction of Chrysanthemum coronarium have a very low hemolysis rate even at high concentrations.
Conclusions: Tests on the essential oil and its oxygenated fraction have revealed promising properties. These results suggest promising opportunities for the development of new agents in the pharmaceutical field.

Keywords

Oxygenated Fraction

Antioxidant Activity

Anti-inflammatory Activity

Hemolytic Effect

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