Association of Mir-149C/T (rs2292832) Polymorphism and Lung Carcinoma Occurrence in the Iranian Population: A Case-Control Study

Torabi, Mehdi; Moeini, Maryam; Kiani, Ebrahim; Sadeghi, Morteza

doi:10.30491/jabr.2023.385605.1605

Association of Mir-149C/T (rs2292832) Polymorphism and Lung Carcinoma Occurrence in the Iranian Population: A Case-Control Study

Document Type : Original Article

Authors

Mehdi Torabi ¹

Maryam Moeini ²

Ebrahim Kiani ¹

Morteza Sadeghi ¹

¹ Human Genetics Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

² Department of Pathology, Chamran Hospital, Tehran, Iran

10.30491/jabr.2023.385605.1605

Abstract

Introduction: Lung carcinoma (LC) is a group of anomalies associated with abnormal respiratory cell divisions. Some studies reported the possible role of the miR-499 (rs3746444) polymorphism in LCs in various societies. In the current case-control study, we decided to investigate the role of miR-149C/T variant on LC occurrence in the Iranian population.
Materials and Methods: In this case-control study, a total of 172 subjects (72 LC patients and 100 control) were genotyped by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) technique, for this aim, 5 ml peripheral blood sample was obtained from each subject and the genomic DNA was extracted by the salting-out method. After genotyping and data collection, the frequency of alleles and genotypes were statistically analyzed (SPSS, v.20).
Results: Since the results showed, in the frequency of all types of genotypes (Dominant, Codominant, Over-dominant, and Recessive) and associated alleles (C and T) in miR149C/T no significant difference (p >0.05) was observed in comparison between the patient group and the control group.
Conclusions: According to the findings of this study there is no significant relation between the miR-499C/T (rs3746444) polymorphism and lung cancer occurrence and this polymorphism is not a significant risk factor for lung cancer in the Iranian population.

Keywords

Carcinoma

Lung,

rs2292832

miR-149C/T

Polymorphism