Journal of Applied Biotechnology Reports

Journal of Applied Biotechnology Reports

Expression Profile of Hyoscyamine Biosynthesis-related Genes in Response to UV-C Radiation in Datura metel Plant

Document Type : Original Article

Authors
Ramin Karimian 1
Milad Nafari 1
Mohammad Reza Khodabakhshi 2
Seyed Javad Davarpanah 2
1 Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
2 Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
10.30491/jabr.2023.377440.1587
Abstract
Introduction: Tropane alkaloids as secondary metabolites are one of the most useful plant elements that are widely applied in medicinal approaches. Studies have shown that UV light led secondary metabolites to be increased. Thus, we investigated the effect of UV-C light on the expression of the main genes involved in the biosynthesis of tropane alkaloids, namely hyoscyamine 6β-hydroxylase (H6H), Putrescine N-methyltransferase (PMT), and Tropinone reductase I (TR-I).
Materials and Methods: Datura metel seeds cultured on MS media at 25 °C less than 12 h-12 h light-dark photoperiod. Then, they were transferred into vases and kept in the greenhouse. Three-month-old plants received 196 µWcm-2 UV-C light for 30 min. Afterward, the expression levels of different genes encoding H6H, PMT, and, TR-I enzymes, were measured at different post-exposure times.
Results: Our results demonstrated that UV-C increased the expression of PMT and TR-I genes after 48 h. Moreover, the rise of H6H expression was found after 24 h but its level was downregulated again after 48 h.
Conclusions: These findings indicated that UV-C light as abiotic stress could boost the formation of tropane alkaloids through upregulation of genes of enzymes catalyzing the main steps in their biosynthesis and, also, these genes are differentially affected. 
Keywords
Datura metel
UV-C
H6H
PMT
TR-I
Hyoscyamine
Journal of Applied Biotechnology Reports
Volume 10, Issue 4
Autumn 2023
Pages 1176-1181

  • Receive Date 20 December 2022
  • Revise Date 06 April 2023
  • Accept Date 19 May 2023