Document Type : Original Article
Department of Food Science and Technology, Faculty of Agriculture, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
Plant Improvement and Seed Production Research Center, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
Department of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Science and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Introduction: Recently, Spirulina platensis has scientifically become popular because of its importance as food, feed, and a natural producer of pigments with specific nutritional and functional characteristics.
Materials and Methods: In this study, the effect of various environmental factors affecting growth conditions of Spirulina platensis, including primary inoculation, light-dark cycle, cultivation time, Light-Emitting Diode (LED) composition, nitrogen source, carbon source, and NaCl concentration, on biomass, C-phycocyanin (C-PC), Allophycocyanin (APC) and chlorophyll-a contents were assessed using Placket-Burman Design (PBD).
Results: Results showed that out of the seven screened factors, four factors of carbon source, LED composition, light-dark cycle and NaCl concentration significantly affected biomass production (p<0.01). Among the investigated factors, nitrogen source, light-dark cycle, and NaCl concentration had significant effects on phycocyanin production (p<0.05). Results showed that cultivation time, light-dark cycle, and NaCl concentration significantly affected the production of allophycocyanin (p<0.05). Furthermore, NaCl concentration, carbon source, LED composition, cultivation time, and initial inoculation included significant effects on chlorophyll-a production (p<0.05).
Conclusions: The present study screened variables affecting biomass, phycocyanin, allophycocyanin, and chlorophyll-a production as the first step in optimizing Spirulina platensis growth condition. Briefly, NaCl concentration was one of the factors which had a significant impact on all responses. The dark cycle also had an effect on three dependent variables except for chlorophyll-a production.