Introduction:The current study aimed to purify antifungal compounds from Chlorella vulgarisextracts, fractions, sub-fractions and pure compounds against different strains of mycotoxigenic fungi. Materials and Methods:Antifungal activity was conducted using disc diffusion assay, TLC-bioautography and Minimum Inhibitory Concentration (MIC). Isolation, purification and structure elucidation of antifungal compounds were carried out using column chromatography, Thin Layer Chromatography (TLC), UV-Vis spectrophotometer, Gas Chromatography–Mass Spectrometry (GC-MS), and Nuclear Magnetic Resonance (NMR). Results:C. vulgarisDiethyl Ether Extract (DEE) showed the highest antifungal activity against all tested fungi with inhibition zone from 11.5 to 21.9 mm. By fractionation of DEE, Fraction F3 (chloroform:methanol, 50:50) and F5 (methanol 100%); sub-fraction CF3-10 and CF5-10 exhibited antifungal activity against all tested fungi. Two pure compounds, hydroxyphenophytin B and hexadecanoic acid methyl ester, with antifungal activity were isolated from CF3-10 and CF5-10, respectively. Conclusions:C. vulgarisDEE and isolated compounds can be used as promising antifungal agents from natural sources against mycotoxigenic fungi at post-harvest or storage stages.
Sultan, Y., & Marrez, D. (2022). Isolation and Purification of Antifungal Compounds from the Green Microalga Chlorella vulgaris. Journal of Applied Biotechnology Reports, 9(2), 603-613. doi: 10.30491/jabr.2021.302307.1438
MLA
Yousef Y. Sultan; Diaa A. Marrez. "Isolation and Purification of Antifungal Compounds from the Green Microalga Chlorella vulgaris". Journal of Applied Biotechnology Reports, 9, 2, 2022, 603-613. doi: 10.30491/jabr.2021.302307.1438
HARVARD
Sultan, Y., Marrez, D. (2022). 'Isolation and Purification of Antifungal Compounds from the Green Microalga Chlorella vulgaris', Journal of Applied Biotechnology Reports, 9(2), pp. 603-613. doi: 10.30491/jabr.2021.302307.1438
VANCOUVER
Sultan, Y., Marrez, D. Isolation and Purification of Antifungal Compounds from the Green Microalga Chlorella vulgaris. Journal of Applied Biotechnology Reports, 2022; 9(2): 603-613. doi: 10.30491/jabr.2021.302307.1438