Influence of Extracellular Vesicles from Lactobacillus rhamnosus GG on the Cell Adhesion and mmp2 and mmp9 Genes Expression in Colorectal Cancer Cells

Parsa, Amir Mohammad; Mahmoodzadeh Hosseini, Hamideh; Mirhosseini, Seyed Ali

doi:10.30491/jabr.2020.249397.1289

Influence of Extracellular Vesicles from Lactobacillus rhamnosus GG on the Cell Adhesion and mmp2 and mmp9 Genes Expression in Colorectal Cancer Cells

Document Type : Original Article

Authors

Amir Mohammad Parsa

Hamideh Mahmoodzadeh Hosseini

Seyed Ali Mirhosseini

Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

10.30491/jabr.2020.249397.1289

Abstract

Introduction: Lactobacillus rhamnosus GG is a probiotic bacterium with anti-cancer and anti-microbial characteristics. In addition, Extracellular Vesicles (EVs) from Lactobacillus rhamnosus GG showed apoptotic impacts on colorectal cancer cells. In this study, we aimed to isolate EVs from Lactobacillus rhamnosus GG (EVL) and identify its effect on cell adhesion and expression of mmp2 and mmp9 genes.
Materials and Methods: HT29 cells were exposed to 16, 32, 64, 128, 256, 512, 1024, and 2048 µg/ml EVL, and the cell viability, cell adhesion, and mRNA gene expression were investigated using the MTT assay, cell adhesion assay, and real-time PCR.
Results: We found that 128, 256, 512, 1024, and 2048 µg/ml of EVL led to attenuated cell viability of HT29 cells (p<0.05). Moreover, the expression of both mmp2 and mmp9 genes and cell adhesion significantly reduced at 1024 and 2048 µg/ml EVL compared to untreated cells (p<0.05).
Conclusions: According to the findings of the present study, it is suggested that EVL could be effective in cancer cell cytotoxicity.

Keywords

Probiotic

Lactobacillus rhamnosus GG

Colorectal Cancer

Cell Adhesion

Extracellular Vesicle