Introduction:Lactobacillus rhamnosusGG is a probiotic bacterium with anti-cancer and anti-microbial characteristics. In addition, Extracellular Vesicles (EVs) from Lactobacillus rhamnosusGG showed apoptotic impacts on colorectal cancer cells. In this study, we aimed to isolate EVs from Lactobacillus rhamnosusGG (EVL) and identify its effect on cell adhesion and expression of mmp2and mmp9genes. Materials and Methods:HT29 cells were exposed to 16, 32, 64, 128, 256, 512, 1024, and 2048 µg/ml EVL, and the cell viability, cell adhesion, and mRNA gene expression were investigated using the MTT assay, cell adhesion assay, and real-time PCR. Results:We found that 128, 256, 512, 1024, and 2048 µg/ml of EVL led to attenuated cell viability of HT29 cells (p<0.05). Moreover, the expression of both mmp2and mmp9genes and cell adhesion significantly reduced at 1024 and 2048 µg/ml EVL compared to untreated cells (p<0.05). Conclusions:According to the findings of the present study, it is suggested that EVL could be effective in cancer cell cytotoxicity.
Parsa, A. M., Mahmoodzadeh Hosseini, H., & Mirhosseini, S. A. (2022). Influence of Extracellular Vesicles from Lactobacillus rhamnosus GG on the Cell Adhesion and mmp2 and mmp9 Genes Expression in Colorectal Cancer Cells. Journal of Applied Biotechnology Reports, 9(2), 576-581. doi: 10.30491/jabr.2020.249397.1289
MLA
Amir Mohammad Parsa; Hamideh Mahmoodzadeh Hosseini; Seyed Ali Mirhosseini. "Influence of Extracellular Vesicles from Lactobacillus rhamnosus GG on the Cell Adhesion and mmp2 and mmp9 Genes Expression in Colorectal Cancer Cells". Journal of Applied Biotechnology Reports, 9, 2, 2022, 576-581. doi: 10.30491/jabr.2020.249397.1289
HARVARD
Parsa, A. M., Mahmoodzadeh Hosseini, H., Mirhosseini, S. A. (2022). 'Influence of Extracellular Vesicles from Lactobacillus rhamnosus GG on the Cell Adhesion and mmp2 and mmp9 Genes Expression in Colorectal Cancer Cells', Journal of Applied Biotechnology Reports, 9(2), pp. 576-581. doi: 10.30491/jabr.2020.249397.1289
VANCOUVER
Parsa, A. M., Mahmoodzadeh Hosseini, H., Mirhosseini, S. A. Influence of Extracellular Vesicles from Lactobacillus rhamnosus GG on the Cell Adhesion and mmp2 and mmp9 Genes Expression in Colorectal Cancer Cells. Journal of Applied Biotechnology Reports, 2022; 9(2): 576-581. doi: 10.30491/jabr.2020.249397.1289