Evaluation of Chlorella sorokiniana Biomass Recovery by Using Different Chemical-based Flocculants

Menegazzo, Mariana Lara; Ribeiro, Dagon Manoel; De Oliveira, Nathanya Nayla; Marques, Otavio Gonçalves Benites; Fonseca, Gustavo Graciano

doi:10.30491/jabr.2021.291113.1405

Evaluation of Chlorella sorokiniana Biomass Recovery by Using Different Chemical-based Flocculants

Document Type : Original Article

Authors

Mariana Lara Menegazzo ¹

Dagon Manoel Ribeiro ¹

Nathanya Nayla de Oliveira ¹

Otavio Gonçalves Benites Marques ¹

Gustavo Graciano Fonseca ²

¹ Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados, Dourados, MS, Brazil

² Faculty of Natural Resource Sciences, School of Business and Science, University of Akureyri, Akureyri, Iceland

10.30491/jabr.2021.291113.1405

Abstract

Introduction: The nature and the concentration of the chemical agents responsible for cell flocculation are the bottlenecks for microalgae recovery. The aim of the present study was to evaluate different chemical-based flocculants for Chlorella sorokiniana flocculation.
Materials and Methods: The biomass recovery efficiency was evaluated by comparing self-flocculation and flocculation with the ferric chloride, sodium hydroxide, aluminum sulfate, and zinc sulfate. After identifying the best flocculating agent, its concentration was varied to determine the optimal condition by rapid agitation followed by sedimentation (0.25 to 1 g/L).
Results: Zinc sulfate was unsuitable for this strain due to an efficiency lower than 40%. Self-flocculation and sodium hydroxide were fairly efficient (48.65% and 58.06%, respectively). Aluminum sulfate produced moderate results (56.27%), but flocculation took a long time to become efficient. Ferric chloride showed the best potential for flocculation, and in the analysis of different concentrations (0.25 to 1 g/L) showed to be fast and efficient (nearly 80% of biomass recovery in 10 min) at a concentration of 0.75 g/L.
Conclusions: All the flocculants tested in this study can be utilized for biomass recovery, except for the zinc sulfate. The procedure was efficient, inexpensive, and contaminant-free for the recovery of C. sorokiniana biomass.

Keywords

Microalgae

Flocculation

Sedimentation

Ferric Chloride