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Kachuei, R., Rezaie, S., Yadegari, M., Safaie, N., Allameh, A., Aref-poor, M., Imani Fooladi, A., Riazipour, M. (2014). Determination of T-2 Mycotoxin in Fusarium strains by HPLC with fluorescence detector. Journal of Applied Biotechnology Reports, 1(1), 38-43.
Reza Kachuei; Sassan Rezaie; Mohammad Hossein Yadegari; Naser Safaie; Abdol-Amir Allameh; Mohammad-Ali Aref-poor; Abbas-Ali Imani Fooladi; Majid Riazipour. "Determination of T-2 Mycotoxin in Fusarium strains by HPLC with fluorescence detector". Journal of Applied Biotechnology Reports, 1, 1, 2014, 38-43.
Kachuei, R., Rezaie, S., Yadegari, M., Safaie, N., Allameh, A., Aref-poor, M., Imani Fooladi, A., Riazipour, M. (2014). 'Determination of T-2 Mycotoxin in Fusarium strains by HPLC with fluorescence detector', Journal of Applied Biotechnology Reports, 1(1), pp. 38-43.
Kachuei, R., Rezaie, S., Yadegari, M., Safaie, N., Allameh, A., Aref-poor, M., Imani Fooladi, A., Riazipour, M. Determination of T-2 Mycotoxin in Fusarium strains by HPLC with fluorescence detector. Journal of Applied Biotechnology Reports, 2014; 1(1): 38-43.

Determination of T-2 Mycotoxin in Fusarium strains by HPLC with fluorescence detector

Article 10, Volume 1, Issue 1, Winter 2014, Page 38-43  XML PDF (275.18 K)
Document Type: Original Article
Authors
Reza Kachuei1; Sassan Rezaie email 2; Mohammad Hossein Yadegari3; Naser Safaie4; Abdol-Amir Allameh5; Mohammad-Ali Aref-poor6; Abbas-Ali Imani Fooladi7; Majid Riazipour8
1Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
2Department of Medical Parasitology and Mycology, Faculty of Public Health, Tehran University of Medial Sciences, Iran
3Department of Medical Mycology, Faculty of Medicine Science, Tarbiat Modares University, Tehran, Iran
4Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
5Department of Biochemistry, Faculty of Medicine Science, Tarbiat Modares University, Tehran, Iran
6Department of Biology, Basic Science Research, Imam Hossein University, Tehran, Iran
7Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
8Department of Medical Parasitology and Mycology, Faculty of Medicine, Baqiyatallah University of Medial Sciences, Iran
Receive Date: 23 January 2014,  Revise Date: 14 March 2013,  Accept Date: 14 March 2014 
Abstract
T-2 toxin is the most poisonous trichothecene produced by Fusarium species especially F.sporotrichioides. T-2 toxin is a biological contaminant in a number of agricultural commodities that can cause severe diseases among humans and animals and even lead to death. The aim of the current study is the analysis of T-2 mycotoxin in Fusarium species by high-performance liquid chromatography (HPLC) combined with florescence detection and derivatization with 1-antroylnitrile (1-AN). Totally, 11Fusariumisolates and reference strains were studied. The isolates were tested for the T-2 toxin production after growing on rice substrate followed by using specific “Multisep 225 Trich Clean up columns” purification. In this study, T-2 toxin production was ranged from 197.05ug/kg to 8503.07ug/kg. This is the first study of T-2 toxin analysis by HPLC-F in Iran.
Keywords
Fusarium; Trichothecene; T-2 toxin; HPLC-F
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