Determination of T-2 Mycotoxin in Fusarium strains by HPLC with fluorescence detector

Document Type: Original Article

Authors

1 Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

2 Department of Medical Parasitology and Mycology, Faculty of Public Health, Tehran University of Medial Sciences, Iran

3 Department of Medical Mycology, Faculty of Medicine Science, Tarbiat Modares University, Tehran, Iran

4 Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

5 Department of Biochemistry, Faculty of Medicine Science, Tarbiat Modares University, Tehran, Iran

6 Department of Biology, Basic Science Research, Imam Hossein University, Tehran, Iran

7 Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

8 Department of Medical Parasitology and Mycology, Faculty of Medicine, Baqiyatallah University of Medial Sciences, Iran

Abstract

T-2 toxin is the most poisonous trichothecene produced by Fusarium species especially F.sporotrichioides. T-2 toxin is a biological contaminant in a number of agricultural commodities that can cause severe diseases among humans and animals and even lead to death. The aim of the current study is the analysis of T-2 mycotoxin in Fusarium species by high-performance liquid chromatography (HPLC) combined with florescence detection and derivatization with 1-antroylnitrile (1-AN). Totally, 11Fusariumisolates and reference strains were studied. The isolates were tested for the T-2 toxin production after growing on rice substrate followed by using specific “Multisep 225 Trich Clean up columns” purification. In this study, T-2 toxin production was ranged from 197.05ug/kg to 8503.07ug/kg. This is the first study of T-2 toxin analysis by HPLC-F in Iran.

Keywords


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