Journal of Applied Biotechnology Reports

Journal of Applied Biotechnology Reports

Determination of T-2 Mycotoxin in Fusarium strains by HPLC with fluorescence detector

Document Type : Original Article

Authors
Reza Kachuei 1
Sassan Rezaie 2
Mohammad Hossein Yadegari 3
Naser Safaie 4
Abdol-Amir Allameh 5
Mohammad-Ali Aref-poor 6
Abbas-Ali Imani Fooladi 7
Majid Riazipour 8
1 Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
2 Department of Medical Parasitology and Mycology, Faculty of Public Health, Tehran University of Medial Sciences, Iran
3 Department of Medical Mycology, Faculty of Medicine Science, Tarbiat Modares University, Tehran, Iran
4 Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
5 Department of Biochemistry, Faculty of Medicine Science, Tarbiat Modares University, Tehran, Iran
6 Department of Biology, Basic Science Research, Imam Hossein University, Tehran, Iran
7 Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
8 Department of Medical Parasitology and Mycology, Faculty of Medicine, Baqiyatallah University of Medial Sciences, Iran
Abstract
T-2 toxin is the most poisonous trichothecene produced by Fusarium species especially F.sporotrichioides. T-2 toxin is a biological contaminant in a number of agricultural commodities that can cause severe diseases among humans and animals and even lead to death. The aim of the current study is the analysis of T-2 mycotoxin in Fusarium species by high-performance liquid chromatography (HPLC) combined with florescence detection and derivatization with 1-antroylnitrile (1-AN). Totally, 11Fusariumisolates and reference strains were studied. The isolates were tested for the T-2 toxin production after growing on rice substrate followed by using specific “Multisep 225 Trich Clean up columns” purification. In this study, T-2 toxin production was ranged from 197.05ug/kg to 8503.07ug/kg. This is the first study of T-2 toxin analysis by HPLC-F in Iran.
Keywords
Fusarium
Trichothecene
T-2 toxin
HPLC-F
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Journal of Applied Biotechnology Reports
Volume 1, Issue 1
Winter 2014
Pages 38-43

  • Receive Date 23 January 2014
  • Revise Date 14 March 2013
  • Accept Date 14 March 2014