@article { author = {Kachuei, Reza and Rezaie, Sassan and Yadegari, Mohammad Hossein and Safaie, Naser and Allameh, Abdol-Amir and Aref-poor, Mohammad-Ali and Imani Fooladi, Abbas-Ali and Riazipour, Majid}, title = {Determination of T-2 Mycotoxin in Fusarium strains by HPLC with fluorescence detector}, journal = {Journal of Applied Biotechnology Reports}, volume = {1}, number = {1}, pages = {38-43}, year = {2014}, publisher = {Baqiyatallah University of Medical Sciences}, issn = {2322-1186}, eissn = {2423-5784}, doi = {}, abstract = {T-2 toxin is the most poisonous trichothecene produced by Fusarium species especially F.sporotrichioides. T-2 toxin is a biological contaminant in a number of agricultural commodities that can cause severe diseases among humans and animals and even lead to death. The aim of the current study is the analysis of T-2 mycotoxin in Fusarium species by high-performance liquid chromatography (HPLC) combined with florescence detection and derivatization with 1-antroylnitrile (1-AN). Totally, 11Fusariumisolates and reference strains were studied. The isolates were tested for the T-2 toxin production after growing on rice substrate followed by using specific “Multisep 225 Trich Clean up columns” purification. In this study, T-2 toxin production was ranged from 197.05ug/kg to 8503.07ug/kg. This is the first study of T-2 toxin analysis by HPLC-F in Iran.}, keywords = {Fusarium,Trichothecene,T-2 toxin,HPLC-F}, url = {https://www.biotechrep.ir/article_68941.html}, eprint = {https://www.biotechrep.ir/article_68941_56d33ed14a114b64f7195cdcf1c68c27.pdf} }