Document Type : Original Article
Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, Iran
Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran
Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
An amperometric glucose biosensor was developed based on synergistic contributions of prussian blue (PB) and a bucky gel (BG) consisting of carbon nanotubes (CNTs) and ionic liquid (IL). The PB nanoparticles were first deposited onto the surface of a BG modified glassy carbon (GC) electrode (BG/GC). Then, the Ni2+ ions were electrochemically inserted into the PB lattice to improve its stability at physiological pH. Afterwards, Glucose oxidase (GOx) was immobilized on the BG/GC electrode using a cross-linking method. Amperometric measurements of glucose were performed at −0.05 V vs. Ag/AgCl in 0.05 M phosphate buffer solution at pH 7.4. The glucose biosensor exhibited a sensitivity of 45.03 µA/(mM.cm2) with a detection limit of 5×10-7 M. The amperometric response was linear in the range of 5×10-7 to 8.3×10−4 M.