Document Type : Review Article
Department of Biology, Damghan Azad University, Damghan, Iran
Department of Biology, Faculty of Science, Imam Hossein University, Tehran, Iran
Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
Due to the spread of infectious diseases, the existence of a rapid and sensitive detection method is necessary today. Polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) is a simple manner for detection of microorganism. For example, bacteria, viruses, fungi and others based on nucleic acid sequence. A large number of samples can be screened by this technique simultaneously, so it is not time consuming and is a quick manner. The high sensitivity and specificity of PCR-ELISA make it a powerful technique by simple laboratory facilities. As a result it can be an excellent substituted manner for analysis and detectionin different various fields.