Introduction:Radiotherapy is a standard and effective modality for breast cancer treatment, through induction of DNA damages notably DNA double-strand breaks which are involved in radiation-induced cell death. All radiation-induced DNA damages are subjected to various repair processes, therefore, interference in the DNA repair pathways might result in radio-resistance. Non-coding RNAs are a diverse group of functional RNA molecules that are not translated into proteins. Recent studies have shown that radiation can cause expression changes in noncoding RNAs. Materials and Methods:MCF-7 and MDA-MB-231 cell lines were grown in a DMEM culture medium supplemented with fetal bovine serum and antibiotics. At exponential growth, cells were exposed to various doses of megavoltage X-rays. 24 and 48 h after irradiation cells were harvested, RNA was extracted and cDNA was synthesized. The expression level of lncRNAs was measured using quantitative real-time PCR. Results:This study showed that radiation could increase DANCRand TUG1lncRNAs expression in breast cancer cell lines 24 and 48 h after receiving radiation. Also, the results suggested that after radiation, the expression of DANCRin the radioresistant cell line was higher than the radiosensitive one; in the case of TUG1, it’s unlike DANCR. Conclusions:The radiation increased the expression of DANCR and TUG1 lncRNAs in breast cancer cell lines because the expression of DANCRin the MDA-MB- 231 was higher than in the MCF-7. In contrast, the expression level of TUG1 in MCF-7 was higher than the MDA-MB-231. Therefore, lncRNAs, DNACR, and TUG1might play a role in the radioresistance and radiosensitivity of breast cancer, respectively.
Nejadtaghi, M., & Mozdarani, H. (2023). The Effect of Radiation on the Expression Level of DANCR and TUG1 LncRNAs in Breast Cancer Cell Lines. Journal of Applied Biotechnology Reports, 10(2), 1009-1017. doi: 10.30491/jabr.2023.366752.1570
MLA
Mahdieh Nejadtaghi; Hossein Mozdarani. "The Effect of Radiation on the Expression Level of DANCR and TUG1 LncRNAs in Breast Cancer Cell Lines". Journal of Applied Biotechnology Reports, 10, 2, 2023, 1009-1017. doi: 10.30491/jabr.2023.366752.1570
HARVARD
Nejadtaghi, M., Mozdarani, H. (2023). 'The Effect of Radiation on the Expression Level of DANCR and TUG1 LncRNAs in Breast Cancer Cell Lines', Journal of Applied Biotechnology Reports, 10(2), pp. 1009-1017. doi: 10.30491/jabr.2023.366752.1570
VANCOUVER
Nejadtaghi, M., Mozdarani, H. The Effect of Radiation on the Expression Level of DANCR and TUG1 LncRNAs in Breast Cancer Cell Lines. Journal of Applied Biotechnology Reports, 2023; 10(2): 1009-1017. doi: 10.30491/jabr.2023.366752.1570
)
