An Effective Method for Extracting DNA From Human Hair Samples

Document Type : Original Article

Authors

1 Human Genetics Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

2 Department of Biology, Payame Noor University, Mashhad, Iran

3 Human Genetics Research Center, Baqiyatallah University of Medical Sciences Tehran, Iran

4 Molecular Detection Laboratory, Valiasr Medical Clinic, Mashhad, Iran

Abstract

IntroductionIn historical cases, mass disasters, missing person’s identification, and Archaeogenetic investigations, the bone, teeth, and hair samples are often the best and the only biological material available for DNA typing. In this study, we demonstrated an extremely effective protocol for DNA extraction of hair.
Materials and Methods: Obtaining genetic information from hair samples for DNA typing involves the following steps: sterilization, DNA extraction, quality control, PCR, and profiling. After extracting aDNA (Ancient DNA) from the hair, autosomal STR analyses were performed for each sample using the AmpFℓSTR® MiniFiler™ and Identifiler™ kits.
Result: Qualitative analysis of DNA by electrophoresis showed the excellent quality of the extracted samples so that the electrophoresed bands were clearly visible.
Conclusions: The results demonstrated that the FDEH method is cheaper and faster than commercial kits; moreover, this new method enhances DNA recovery from hair. Because of simple protocol and high quality, this method can be utilized in Medical.

Keywords



Articles in Press, Accepted Manuscript
Available Online from 13 October 2020
  • Receive Date: 10 August 2019
  • Revise Date: 10 December 2019
  • Accept Date: 28 December 2019