Document Type: Original Article
Human Genetics Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Introduction: MicroRNAs (miRNAs) play an important role in the expression of their target genes. The Single-Nucleotide Polymorphisms (SNPs) in miRNAs may affect their function and expression. The aim of the present study was to investigate the association between miR-196a2 rs116614913 polymorphism and Non-Small Cell Lung Cancer (NSCLC) in the Iranian population.
Materials and Methods: This case-control study was performed among 103 lung cancer patients and 100 healthy controls. The Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method and direct sequencing were used for miR-196a2 polymorphism genotyping. Statistical analyses were performed using SPSS software and the t-test method.
Results: According to findings of this study, there was no significant association between rs11614913 polymorphisms and the risk of lung cancer in the codominant model (CT vs.CC: OR=0.67, TT vs.CC: OR=0.74, CT + CC vs. CC: OR=1.133), dominant model (CT+TT vs. CC: OR=0.657) and recessive model (TT vs. CC+CT: OR=0.88). In addition, here was no relationship between the clinicopathological characteristics of patients and controls.
Conclusions: In summary, findings indicated no significant association between miR-196a2 rs11614913 polymorphisms and lung cancer in the Iranian population. Further studies with larger sample sizes are recommended to verify these findings.