Investigation of the Association Between a Genetic Variant in MiR-196a-2 Gene and the Risk of Lung Cancer in the Iranian Population

Document Type: Original Article


1 Human Genetics Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

2 Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran



Introduction: MicroRNAs (miRNAs) play an important role in the expression of their target genes. The Single-Nucleotide Polymorphisms (SNPs) in miRNAs may affect their function and expression. The aim of the present study was to investigate the association between miR-196a2 rs116614913 polymorphism and Non-Small Cell Lung Cancer (NSCLC) in the Iranian population.
Materials and Methods: This case-control study was performed among 103 lung cancer patients and 100 healthy controls. The Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method and direct sequencing were used for miR-196a2 polymorphism genotyping. Statistical analyses were performed using SPSS software and the t-test method.
Results: According to findings of this study, there was no significant association between rs11614913 polymorphisms and the risk of lung cancer in the codominant model (CT vs.CC: OR=0.67, TT vs.CC: OR=0.74, CT + CC vs. CC: OR=1.133), dominant model (CT+TT vs. CC: OR=0.657) and recessive model (TT vs. CC+CT: OR=0.88). In addition, here was no relationship between the clinicopathological characteristics of patients and controls.
Conclusions: In summary, findings indicated no significant association between miR-196a2 rs11614913 polymorphisms and lung cancer in the Iranian population. Further studies with larger sample sizes are recommended to verify these findings.


Articles in Press, Accepted Manuscript
Available Online from 04 July 2020
  • Receive Date: 22 April 2019
  • Revise Date: 10 August 2019
  • Accept Date: 11 August 2019