Document Type : Original Article
Department of Anatomical Sciences and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran
Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Department of Reproductive Biology, Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Introduction: Avocado and soya unsaponifiables (ASU) have been reported to be useful for the treatment of cartilage diseases. The aim of this study was to detect whether avocado/soybean can have any effect on the chondrogenesis of human adipose-derived stem cells on polylactic-co-glycolic acid/fibrin hybrid scaffold or not.
Materials and Methods: The poly-lactic-co-glycolic acid (PLGA)/fibrin scaffolds were seeded with cultured human adipose tissue-derived stem cells (hADSCs), which were then divided into three groups: control, TGF-β3, and ASU and the results were analyzed 14 days later. The viability of the cells in different groups were assessed by MTT. The expression of chondrogenic-related genes Sox9, type II collagen, Aggrecan, type X collagen, and type I collagen were quantified by real time polymerase chain reaction (PCR). Protein expression levels of collagen type II and X were evaluated by Western blotting.
Results: Enhanced cellular viability was observed in the ASU group compared to the transforming growth factor beta-3 (TGF-β3) group. Analysis of aggrecan (Agg), type II collagen (Coll2) and SOX9 revealed that ASU and TGF-β3 induce hADSCs on PLGA/fibrin scaffold to differentiate into chondrocytes in-vitro. Moreover, a significant decrease was observed in the expression of type X (Coll10) and I collagen (Coll1) genes in the ASU group compared to the TGF-β3 group. Protein levels of type II collagen (Coll2) significantly increased in TGF-β3 and ASU groups in comparison with those of the control group. However, protein levels of Type X collagen (Coll10) significantly declined in the ASU group when compared with the TGF-β3 group.
Conclusions: The results of the present study indicated that hADSCs containing the ASU in PLGA/fibrin hybrid scaffold are an effective way to potentially enhance Cartilage-specific genes with less hypertrophy and Fibrosis in-vitro.