Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 3 3 2016 09 01 The Type I and II Secretory Systems in Gram-Negative Bacteria: A Brief Overview 437 440 69228 EN Jafar Khezri National Institute of Genetic Engineering and Biotechnology Iran, Tehran Fathollah Ahmadpour Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Samira Talebi National Institute of Genetic Engineering and Biotechnology Iran, Tehran Journal Article 2016 09 15 Bacteria utilize a multitude of methods to secretion of specific substrate across phospholipid membranes. These bacterial strategies can play many roles in promoting bacterial process, from enhancing pathogenesis to bacterial response to environmental condition. The secretion in bacterial species transfer and release performance substance such as proteins, enzymes and metabolites to environment. The process of secretion plays an important role in the performance and compatibility with environment. Many bacteria use dedicated protein secretion systems to secrete from the cytosol into environment. In general, bacterial protein secretion systems classified to different classes, there are at least six specific secretory systems in Gram-negative bacteria. In this study, we review type I and II secretory systems of Gram-negative bacteria.

https://www.biotechrep.ir/article_69228_dd6978566d09bd475c5a6f3e0a4d6d18.pdf

Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 3 3 2016 09 01 Assessment of Genetic Diversity by Application of Inter Simple Sequence Repeat (ISSR) Primers on Iranian Harmal (Peganum harmala L.) Germplasm as an Important Medicinal Plant 441 445 69229 EN Alireza Zebarjadi Agronomy and Plant Breeding Department, Faculty of Agriculture, Razi University, Kermanshah, Iran Biotechnology for Drought Tolerance Research Department, Razi University, Kermanshah, Iran 0000-0002-7091-3847 Hossein Rostami Ahmadvandi Agronomy and Plant Breeding Department, Faculty of Agriculture, Razi University, Kermanshah, Iran Danial Kahrizi Agronomy and Plant Breeding Department, Faculty of Agriculture, Razi University, Kermanshah, Iran Biotechnology for Drought Tolerance Research Department, Razi University, Kermanshah, Iran 0000-0002-1717-6075 Kianoosh Cheghamirza Agronomy and Plant Breeding Department, Faculty of Agriculture, Razi University, Kermanshah, Iran Biotechnology for Drought Tolerance Research Department, Razi University, Kermanshah, Iran Journal Article 2016 09 15 Syrian Rue or Harmal (Peganum harmala L.), belonged to the family of Peganaceae, grows in semi-arid climates such as the Middle East and North Africa. Traditionally, this plant, especially the seeds, has been recognized for its several medicinal uses. In this stydy, genetic diversity between 21 Harmal accessions, collected from different regions of Iran were evaluated by Inter Simple Sequence Repeat (ISSR) marker using 14 spesific primers. All primers successfully amplified polymorphism regions, as among 115 regions, 68 polymorphic regions (59.13%) were amplified. Accordingly, highest and lowest similarities among ecotypes were measured as 94% and 55%, respectively. The results based on cluster analysis also categorized all accessions into three groups that did not completely match to their geographic pattern place of collection. Result of principle coordinate analysis of samples also showed distribution pattern similar to cluster analysis.

https://www.biotechrep.ir/article_69229_dc346f5dee286bd3faa7645724ebf79f.pdf

Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 3 3 2016 09 01 Isolating Two Native Extreme Halophilic Bacterial Strains Producing Bacteriorhodopsin Protein from Aran-Bidgol Lake 447 452 69230 EN Sahar Shakuri Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Ali Mohammad Latifi Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran 0000-0002-8952-5174 Morteza Mirzaei Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Samaneh Khodi Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Journal Article 2016 09 15 Bacteriorhodopsin operates as a light/proton transfer pump which converts the light energy into a proton gradient. The energy stored in the proton gradient can be used in a variety of ways. The main source of Bacteriorhodopsin are some Halobacterium species such as Halobacterium sodomense and Halobacterium salinarum which grow in harsh and salt-saturated conditions. In order to isolate strains from Aran-Bidgol Lake, two red pigment (IRLS.1) and orange pigment (IRLS.2) strains were isolated. Spectroscopy reviews and the results of SDS-Page of membrane proteins of two isolated strains as well as Iranian native Halobacterium salinarum showed that bacteriorhodopsin protein presents in the collected sample. Spectroscopic studies showed that Halobacterium salinarum produces the maximum amount than IRLS.1 and IRLS.2 produces less and lesser amount of bacteriorhodopsin respectively. The results of biochemical and molecular identification based on the 16srRNA of both mentioned strains indicated their highest similarity with Natrinema sp. XA3-1 and Archaeon RC34, respectively. In this study, the presence of bacteriorhodopsin protein in Iranian native strains was examined for the first time and the local strains were isolated purely from Halobacterium salinarum membrane by gel filtration chromatography that given the widespread use of bacteriorhodopsin protein, it will be so effective.

https://www.biotechrep.ir/article_69230_7fef78c47060974e0b8392e305f0daf0.pdf

Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 3 3 2016 09 01 The Biological Effects of Teucrium polium on the Severity of Primary Dysmenorrhea 453 456 69232 EN Khadige Abadian School of Nursing and Midwifery, International branch of Shahid Beheshti University of Medical Sciences, Tehran, Iran Zohre Keshavarz Department of Midwifery and Reproductive Health, School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, Iran Faraz Mojab Department of Pharmacogenosy, Faculty of Pharmacology, Shahid Beheshti University of Medical Sciences, Tehran, Iran Neda Bromand Department of Biostatistics, Faculty of Paramedicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Journal Article 2016 09 15 Primary dysmenorrhea is a prevalent issue and it can reduce the quality of life in many females worldwide. Based on the properties of Teucrium polium as a herbal medicine with analgesic and inflammatory properties, this triple-blind, randomized, placebo controlled trial was performed. The study aimed to evaluate the effects of Teucrium polium on the severity of primary dysmenorrhea in students. In this study, single students were categorized in two groups who received Teucrium Polium (n=35) or placebo (n=35). For the first 3 days of menstruation, subjects were asked to take some capsules containing powder Teucrium polium (250 mg) four times daily for two consecutive menstrual cycles. Pain severity was examined using a visual analog scale. According to the results, there was no significant difference in the pain severity at the baseline between two groups. Pain severity was significantly decreased in both groups after the intervention; however, larger pain reduction was significantly seen in the group which had received Teucrium Polium (p< 0.001). No side effect was observed in the Teucrium Polium-received group. In conclusion feature of Teucrium polium during menstruation led to decrease in the severity of dysmenorrhea.

https://www.biotechrep.ir/article_69232_daef3dd225154d7a50df068cdc4c07b8.pdf

Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 3 3 2016 09 01 Feasibility of Sunflower (Helianthus annus L.) Plantation in Low to Moderately Contaminated Brownfields to Achieve Remediation Objectives 457 463 69233 EN Ali Daryabeigi Zand Graduate Faculty of Environment, University of Tehran, Tehran, Iran 0000-0002-2490-0893 Hassan Hoveidi Graduate Faculty of Environment, University of Tehran, Tehran, Iran Journal Article 2016 09 15 Phytoremediation has gained increasing attention in recent years because of its ability to clean up and redevelop brownfield sites. Petroleum hydrocarbon-contaminated sites are among most prevalent industrial sites in Iran as one of the top oil producers in the world, which are in great need of remediation to prevent associated environmental and human health risks. A greenhouse study was conducted to identify the tolerance of sunflower (Helianthus annus L.) and its growth parameters under various scenarios. Additionally, remediation potential of sunflower in spiked soil with low to moderately gasoline-contaminated soil was evaluated. Results indicated that sunflower is a tolerant plant species in low to moderately gasoline-contaminated soil with remarkable biomass establishment in contaminated soil and acceptable phytoremediation potential. Sunflower was found to tolerate gasoline contamination up to the maximum applied concentration of 5000 mg/kg. However, sunflower biomass and height slightly decreased in presence of gasoline in soil which were not significant in most cases (P>0.05). Significant reduction of gasoline under the influence of sunflower was obtained in all vegetated treatments when compared to non-vegetated treatments (P<0.05). Phytoremediation effectiveness of sunflower did not vary significantly with increasing contamination level in soil (P>0.05). Results of the present research indicated that gasoline content of soil cannot be considered as a controlling factor affecting phytoremediation potential of sunflower when soil is polluted with low to moderate levels of gasoline. Soil remediation with sunflower can be considered as a promising approach to manage moderately gasoline-contaminated sites.

https://www.biotechrep.ir/article_69233_3e15dd60619135f202070e3aaec94f7b.pdf

Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 3 3 2016 09 01 DNA Molecular Markers Depicted Genetic Variability and Heterotic Pattern Among Maize Inbred Lines 465 472 69234 EN Fahimeh Mohamadi Suni Agronomy and Plant Breeding Department, Ramin Agriculture and Natural Resources University of Khuzestan, Mollasani, Ahvaz, Iran Mohammad Farkhari Agronomy and Plant Breeding Department, Ramin Agriculture and Natural Resources University of Khuzestan, Mollasani, Ahvaz, Iran Henghameh Taheri Agronomy and Plant Breeding Department, Ramin Agriculture and Natural Resources University of Khuzestan, Mollasani, Ahvaz, Iran Payam Pour Mohammadi Agronomy and Plant Breeding Department, Ramin Agriculture and Natural Resources University of Khuzestan, Mollasani, Ahvaz, Iran Mohammad Barzegari Safiabad Agricultural Research Center, Khouzestan, Dezful, Iran Journal Article 2016 09 15 Crosses between lines with suitable genetic distance could lead to excellent hybrids, so the identification of genetic diversity between Zea mays L. inbred lines has a high priority in maize hybrid breeding programs. Here, fingerprinting of 27 maize inbred lines accompanied with lines B73 and Mo17 (as parents of commercial hybrid Sc704) were evaluated via15 Inter-Simple Sequence Repeat (ISSR) primers. A total of 127 bands were amplified which 107 (85.5%) out of them were polymorphic with an average PIC of 33% (between 0.44 for UBC-820 to 0.22 for ISSR-11). Classification of genotypes based on simple matching similarity coefficients and furthest neighbor (complete linkage) clustering algorithm located them into three distinguished groups. In spite of narrow information about pedigree of studied lines, results of present study expressed the existence of at least two heterotic groups in this germplasm. These findings could be applied in management and utilization of these studied lines for developing new maize hybrid.

https://www.biotechrep.ir/article_69234_c4e7bc3145caf05a5689ed8d5cf1eb36.pdf

Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 3 3 2016 09 01 Development of PCR-Based Method for Rapid Detection of Abrin Gene 473 476 69235 EN Morteza Hamedi Behnaq Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Ali Karami Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Ali Choopani Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Department of Biology, Payam Noor University, Isfahan, Iran 0000-0002-9072-5085 Journal Article 2016 09 15 Abrin, known as a ribosome inactivating protein (RIP), is a high cytotoxic plant protein. The high lethality, low cost, and easy access to this plant and its seeds have led to this toxin to be used in crimes and terrorist acts. Since, obtaining purified toxins requires advanced laboratory equipment and complex procedures, it seems that the perpetrators of such crimes use crude extracts. As a result, it was hypothesized that remaining the specific toxin genes in these extracts can provide the advantage of using PCR assay to identify abrin gene which refers to the existence of its toxin. We used a new rapid molecular method for the detection of the abrin gene by PCR. In this regard, specific primers were designed and the required DNA was extracted from Rosary pie samples using cetyltrimethylammonium bromide-polyvinylpyrrolidone (CTAB-PVP) method and PCR protocol was performed using specific primers. Then, assay’s sensitivity was analyzed using serial dilution method. The results of this study revealed that designed and selected primers sequence for toxin’s gene function as specific. The desired product size was obtained and sequencing of PCR products showed up to 90% similarity with known sequence for each molecule. According to these results, the developed rapid molecular method for detection of abrin toxin gene can be considered as a sensitive and low-cost detection method for this toxin gene in cases of suspection to bioterrorism event.

https://www.biotechrep.ir/article_69235_afa2575a14d679e15f18be692bf3ee44.pdf