Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 5 2 2018 06 01 Isolation and Characterization of GDP-L-Galactose Phosphorylase Gene of Vitamin C Biosynthesis Pathway from Kiwi 59 63 74249 10.29252/jabr.05.02.04 EN Soraya Shiri Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran Alireza Zebarjadi Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran 0000-0002-7091-3847 Journal Article 2018 01 25 <strong>Introduction</strong>: Vitamin C is a major antioxidant in plants and plays an important role in reducing the activity of reactive oxygen species. In humans, the main role of this molecule is the elimination of activity of active oxygen species along with being cofactor for many enzymes. Human is one of the few mammalian species that can not synthesize this vitamin and needs to get it through food sources. The GDP-L-galactose phosphorylase (<em>GGP</em>) gene is one of the most important genes in the biosynthetic pathway of vitamin C, which codes for the GDPL-galactose phosphorylase enzyme. Isolation of <em>GGP </em>gene is an important step in transferring it to elevate vitamin C biosynthesis in plants.<br /> <strong>Materials and Methods</strong>: In current study, the isolation of this gene from kiwi plant was carried out and then was cloned in the pTG19-T plasmid via T/A cloning and subsequently sequenced to confirm it.<br /> <strong>Results</strong>: Sequencing analysis of the <em>GGP </em>gene showed that this fragment contains 1383 bp and the start and stop codons were ATG and TGA, respectively. The bioinformatics analysis of this gene can provide important information on gene and protein structure. The alignment of cloned sequence was done with other <em>Actinidia </em>DNA sequences. The results based on neighbor-joining alignment showed that some of the mutations in nucleotides were related to the third nucleotide in a specific codon. Also, the minimum distance of protein sequences was observed between isolated <em>GGP </em>and <em>Actinidia chinensis</em>.<br /> <strong>Conclusions</strong>: Based on analyses, isolated gene (<em>GGP</em>) can be used for increase vitamin C content in other plants such as cucumber and for resistance to environmental stresses in different plants. https://www.biotechrep.ir/article_74249_7f9d5084b8246feed7719e125b3834f6.pdf