Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 3 4 2016 12 01 Enzymatic and Non-enzymatic Antioxidants of Calamintha officinalis Moench Extracts 489 494 69239 EN Fatemeh Shams Moattar Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Reyhaneh Sariri Department of Chemistry, Islamic Azad University, Lahijan Branch, Lahijan, Iran Parichehr Yaghmaee Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Masoud Giahi Department of Chemistry, Islamic Azad University, Lahijan Branch, Lahijan, Iran Journal Article 2016 09 16 <em>Calamintha officinalis</em> (COM) is a genus of aromatic herbs, native in north of Iran. Partial purification of aqueous extract from COM was done using ammonium sulfate precipitation followed by dialysis. The total protein content was determined at various purification stages. The highest amount of protein of the extract was found in fraction obtained in 20-40% saturation range. Specific activities of three antioxidant enzymes, peroxidase, catalase, and superoxide dismutase were also determined and compared in each stage. The highest activity of peroxidase, catalase and  superoxide dismutase was in fractions 60-80%, 20-40%, and 20-40% respectively. The non-enzymatic antioxidants were evaluated by two different <br /> methods i.e. free radical scavenging activity using DPPH and inhibition of lipid peroxidation by the ferric thiocyanate method. It was found that radical scavenging power of COM methanolic extract was 43.99. Furthermore, the inhibition of li<br /> pid peroxidation of the extract was decreased every 24 h. Thus, our findings suggested that <em>C. officinalis</em> possess potent antioxidant defense machinery both enzymatic and non enzymatic. https://www.biotechrep.ir/article_69239_e5124d49e221125e730136d5b2c22112.pdf