Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 2 4 2015 12 01 Computer Aided Design of a Luciferase Like Haloalkane Dehalogenase Enzyme by Homology Based Rational Protein Design (HRPD) Method 315 323 69197 EN Raghunath Satpathy School of Life science, Sambalpur University, Jyoti vihar, Burla, Odisha, India 0000-0001-5296-8492 V. Badireenath Konkimalla School of Biological Sciences, National Institute of Science Education and Research (NISER), Bhubaneswar, Odisha, India Jagnyeswar Ratha School of Life science, Sambalpur University, Jyoti vihar, Burla, Odisha, India Journal Article 2015 09 15 Rational protein design is an important aspect to introduce novel characters to the enzymes. In this report, we describe a procedure for in-silico design of a novel haloalkane dehalogenase protein that exhibits luciferase property, which can be potentially used in biosensor applications. The haloalkane dehalogenase have a close structural homology with a lucifearse was used as the starting structure for design. Amino acids that are frequently interacts with the chromophre (colentrizine) was analyzed by docking and molecular dynamics simulation. The amino acids Asp170, Lys192, Arg193, Lys259, and Lys261 were selected in the enzyme structure for substitution purpose to generate mutant enzyme. Following several selection strategies, it was observed that the protein substituted with Phe in all the positions is the best one which was further validated by a 10 ns molecular dynamics simulation. The designed protein is then analyzed for its substrate specificity towards 10 selected toxic haloalkane compounds. The result shows, that the designed protein has also improved the substrate specificity towards four toxic pollutants. https://www.biotechrep.ir/article_69197_2d6c1d880827eda2e2c932abf9a7102f.pdf