Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 2 2 2015 06 01 Comparison of Extraction Different Methods of Sodium Alginate from Brown Alga Sargassum sp. Localized in the Southern of Iran 251 255 69183 EN Ali Mohammad Latifi Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran 0000-0002-8952-5174 Ehsan Sadegh Nejad Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Hamid Babavalian Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Journal Article 2015 04 15 The alginate was extracted with six different methods from Iran south seacoast algae, <em>Sargassum</em> sp, and the percentage yield of alginate was determined. We divided our methods to two groups including acidic extraction and non-acidic. In acidic methods, HCL and H₂SO₄ were used as a detergent in extraction process and CaCl₂ was exerted in non-acidic treatments. All treatments compared with each other and indicated an increasing in alginate yield when different methods used EDTA in extraction process. Finally, the main characteristics of sodium alginate were realized with FT-IR and H-NMR. https://www.biotechrep.ir/article_69183_241982732a61f33f16414e0d94a49e22.pdf