Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 4 2 2017 06 01 Novel PCR-ELISA Technique as a Good Substitute in Molecular Assay 567 672 68984 EN Fatemeh Tayebeh Department of Biology, Damghan Azad University, Damghan, Iran Shahram Nazarian Department of Biology, Faculty of Science, Imam Hossein University, Tehran, Iran Seyed Ali Mirhosseini Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran Jafar Amani Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran 0000-0002-5155-4738 Journal Article 2017 06 11 Due to the spread of infectious diseases, the existence of a rapid and sensitive detection method is necessary today. Polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) is a simple manner for detection of microorganism. For example, bacteria, viruses, fungi and others based on nucleic acid sequence. A large number of samples can be screened by this technique simultaneously, so it is not time consuming and is a quick manner. The high sensitivity and specificity of PCR-ELISA make it a powerful technique by simple laboratory facilities. As a result it can be an excellent substituted manner for analysis and detectionin different various fields. https://www.biotechrep.ir/article_68984_72ef19939d164082d123f0dea7638bae.pdf