Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 11 1 2024 03 01 Evaluation of the Protective Effect of Organophosphorus Hydrolase on SH-SY5Y Human Neuroblastoma Cells Treated with Parathion 1236 1244 193563 10.30491/jabr.2024.193563 EN Morteza Mirzaei Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran 0000-0001-5568-2422 Mehrdad Moosazadeh Moghaddam Tissue Engineering and Regenerative Medicine Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran 0000-0002-4645-8661 Rezvan Seidmoradi Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Mona Rastegar Shariat Panahi Department of Biology, Payame Noor University, Tehran, Iran Ali Mohammad Latifi Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran 0000-0002-8952-5174 Journal Article 2024 04 06 <span class="fontstyle0"><strong>Introduction:</strong> </span><span class="fontstyle2">Organophosphorus (OPs) compounds are chemical compounds used in pesticides that contain synthetic esters, amides, and thiol derivatives of phosphoric, and phosphonic acids. The OPs are harmful to humans and animals because of compounds such as parathion. By acting on nerve cells, parathion creates very dangerous cellular oxidative stresses, which in turn activate programmed cell death.<br /></span><span class="fontstyle0"><strong>Materials and Methods:</strong> </span><span class="fontstyle2">In this study, the enzyme Organophosphorus Hydrolase (OPH) having esterase activity was selected with the aim of influencing its reaction product with parathion on the viability of human nerve cells. The neuroblastoma SH-SY5Y cell line was exposed to parathion (700 µg/ml) (</span><span class="fontstyle3">≈ </span><span class="fontstyle2">40% reduced cell viability) and the product of OPH esterase reaction (1 µg/ml) with the same parathion concentration for two hours to determine their cytotoxicity (</span><span class="fontstyle3">≈ </span><span class="fontstyle2">25% reduced cell viability) by MTT, real-time PCR and flow cytometry techniques.<br /></span><span class="fontstyle0"><strong>Results:</strong> </span><span class="fontstyle2">The results revealed that parathion (100 µg/ml) inhibited acetylcholinesterase activity by </span><span class="fontstyle3">≈ </span><span class="fontstyle2">65% while OPH-related product reduced acetylcholinesterase activity by </span><span class="fontstyle3">≈ </span><span class="fontstyle2">26%.<br /></span><span class="fontstyle0"><strong>Conclusions:</strong> </span><span class="fontstyle2">Considering the widespread use of OPs in modern agriculture, the OPH can be used to reduce the OPs’ destructive effects and the current study could provide new insight into healthy modern agriculture.</span>  https://www.biotechrep.ir/article_193563_a4f82a026e559b0359f56add28179562.pdf