Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 10 2 2023 06 01 The Effect of Radiation on the Expression Level of DANCR and TUG1 LncRNAs in Breast Cancer Cell Lines 1009 1017 174241 10.30491/jabr.2023.366752.1570 EN Mahdieh Nejadtaghi Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran 0000-0001-8919-2149 Hossein Mozdarani Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Journal Article 2022 10 22 Introduction: Radiotherapy is a standard and effective modality for breast cancer treatment, through induction of DNA damages notably DNA double-strand breaks which are involved in radiation-induced cell death. All radiation-induced DNA damages are subjected to various repair processes, therefore, interference in the DNA repair pathways might result in radio-resistance. Non-coding RNAs are a diverse group of functional RNA molecules that are not translated into proteins. Recent studies have shown that radiation can cause expression changes in noncoding RNAs. Materials and Methods: MCF-7 and MDA-MB-231 cell lines were grown in a DMEM culture medium supplemented with fetal bovine serum and antibiotics. At exponential growth, cells were exposed to various doses of megavoltage X-rays. 24 and 48 h after irradiation cells were harvested, RNA was extracted and cDNA was synthesized. The expression level of lncRNAs was measured using quantitative real-time PCR. Results: This study showed that radiation could increase DANCR and TUG1 lncRNAs expression in breast cancer cell lines 24 and 48 h after receiving radiation. Also, the results suggested that after radiation, the expression of DANCR in the radioresistant cell line was higher than the radiosensitive one; in the case of TUG1, it’s unlike DANCR. Conclusions: The radiation increased the expression of DANCR and TUG1 lncRNAs in breast cancer cell lines because the expression of DANCR in the MDA-MB- 231 was higher than in the MCF-7. In contrast, the expression level of TUG1 in MCF-7 was higher than the MDA-MB-231. Therefore, lncRNAs, DNACR, and TUG1 might play a role in the radioresistance and radiosensitivity of breast cancer, respectively.

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