Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 8 2 2021 06 01 Optimization of Coenzyme Q10 Production by Gluconobacter japonicus FM10 Using Response Surface Methodology 172 179 130940 10.30491/jabr.2021.130940 EN Foozieh Moghadami Department of Biology, Payame Noor University, Tehran, Iran 0000-0001-8751-3535 Ramin Hosseini Department of Biotechnology, Faculty of Agriculture and Natural Resources, Imam Khomeini International University, Qazvin, Iran Jamshid Fooladi Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran 0000-0002-6179-3257 Mahdi Kalantari Department of Statistics, Payame Noor University, Tehran, Iran 0000-0003-4951-1402 Journal Article 2020 01 28 <strong>Introduction:</strong> Coenzyme Q₁₀ is one of the antioxidants with a worldwide market. Nowadays the coenzymeQ₁₀ production has been considered by fermentation using microorganisms. In this study, the Response Surface Methodology was used to optimize culture composition for coenzyme Q₁₀ production by a previously isolated bacterium, <em>Gluconobacter japonicus</em> FM10.<br /> <strong>Materials and methods:</strong> A central composite design was employed to optimize the culture composition including sorbitol, yeast extract, peptone, KH₂PO₄, and MgSO₄ for coenzyme Q₁₀ production. The dry cell weight and coenzymeQ₁₀ concentration were monitored as response variables and the desirability function approach was applied to obtain the optimum level for each factor.<br /> <strong>Results:</strong> Results showed that an average, 3 mg/L of coenzyme Q₁₀ was obtained when the optimized culture composition was employed (110 g/L of sorbitol, 25 g/L of yeast extract, 35 g/L of peptone, 0.5 g/L of KH₂PO_4,and 0.55 g/L of MgSO₄). In addition, the expected dry cell weight reached 6 g/L in the presence of 90 g/L of sorbitol, 17.5 g/L of yeast extract, 35 g/L of peptone, 0 g/L of KH₂PO₄, and 1.7 g/L of MgSO₄.<br /> <strong>Conclusions:</strong> The results of regression analysis revealed that the concentrations of peptone and sorbitol were the most effective factors in producing coenzyme Q₁₀ and dry cell weight, respectively.  https://www.biotechrep.ir/article_130940_32b79ff7893684fc2dd0e8b278617ea3.pdf