Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 7 4 2020 12 01 Encapsulation of Essential Oils of Mentha pulegium and Ferula gummosa Using Nanoliposome Technology as a Safe Botanical Pesticide 237 242 121704 10.30491/jabr.2020.121704 EN Zahra Faraji Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran Jahanshir Shakarami Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran 0000-0003-3948-4113 Jaleh Varshosaz Department of Pharmaceutics, School of Pharmacy and Novel Drug Delivery Systems Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran Shahriar Jafari Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran Journal Article 2019 12 27 <strong>Introduction:</strong> Recent researches have shown that many plant Essential Oils (EOs) have a high potential for controlling agricultural pests and can be used as precursors for synthesis of new pesticides. The major limitations for the use of these compounds are rapid evaporation, poor water solubility, and aptitude for oxidation. The aim of this study was to prepare and characterize nanoliposome containing EOs of <em>Mentha</em> <em>pulegium</em> and <em>Ferula gummosa</em> and fumigant toxicity of nanoliposome containing <em>M. pulegium </em>EOagainst <em>T. castaneum.</em><br /> <strong>Materials and Methods:</strong> In this study, nanoliposome containing EOs of <em>M. pulegium </em>and<em> F. gummosa </em>were prepared using heating method and its physicochemical properties were evaluated. Also, the impact of fumigant toxicity of <em>M. pulegium </em>EONanoliposome on <em>M</em>. <em>castaneum</em> was investigated.<br /> <strong>Results:</strong> Results showed that mean (±SD) particles of nanoliposomecontaining<em> M. pulegium</em> and <em>F. gummosa</em> EOs were 345±3.2 and 309±1.67 nm and their encapsulation efficiency were 99.38±0.24% and 96.41±0.26, respectively. The kind of EOs had no significant effect on the physicochemical property of nanoparticles. At the end of 24 h, the release percentage of EOs of nanoliposomes of<em> M. Pulegium </em>and<em> F. gummosa</em> were 46% and 33 %, respectively. The estimated LC₅₀ values for nanoliposome and crude Eos of <em>M. Pulegium </em>against <em>T. castaneum </em>were36.53 and 75.23 µI/I air, respectively.<br /> <strong>Conclusions:</strong> The results of the current research showed that release and stability of EOs were significantly affected when change to nanoliposome particles. Also, <em>M. pleugium</em> EO nanoliposome showed enhancing fumigant toxicity against <em>T. castaneum</em> in comparison with the crude EO of this plant. https://www.biotechrep.ir/article_121704_ae8f7faa0f65e368939000184758c502.pdf