Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 6 4 2019 12 05 The Antibacterial Effects of the Mixture of Silver Nanoparticles With the Shallot and Nettle Alcoholic Extracts 158 164 100749 10.29252/JABR.06.04.05 EN Sepideh Zeinali Aghdam Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran Antimicrobial Resistance Research Center, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran Sara Minaeian Antimicrobial Resistance Research Center, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran Meisam Sadeghpour Karimi Center of Excellence in Electrochemistry, University of Tehran, Tehran, Iran Akram Sadat Tabatabaee Bafroee Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran 0000-0003-0309-2206 Journal Article 2019 02 16 <strong>Introduction</strong>: Today, one of the most important challenges of the therapeutic system is the resistance of bacteria against different antibiotics especially in intensive care units which lead to an increase in hospitalization time and the patients’ expenses. <em>Acinetobacter baumannii </em>is one of the most significant contaminating bacteria in intensive care units which has exhibited resistance against different antibiotics in recent years. The aim of this study was to investigate the synergism effect of the silver nanoparticles with the shallot and nettle alcoholic extracts against the standard and multidrug resistant <em>A. baumannii </em>isolates.<br /> <strong>Materials and Methods: </strong>Samples were collected from intensive care units and the <em>A. baumannii </em>isolates were identified using biochemical tests. Then, the antibiogram test was carried out for each isolate. The antibacterial effect of nanoparticles, shallot and nettle extracts was evaluated singularly and in combination with each other against standard and resistant <em>A. baumannii </em>isolates. Measuring the diameter of inhibited growth zone, MIC, MBC and checkerboard tests were conducted for each isolate.<br /> <strong>Results: </strong>The results showed that the silver nanoparticles, shallot and nettle alcoholic extracts each had antibacterial property against the standard and resistant <em>A. baumannii </em>isolates. The mixture of the nettle extract with silver nanoparticles had a synergism effect against the standard and resistant isolates and the mixture of the shallot extract with silver nanoparticles had an additive effect against <em>A. baumannii </em>isolates.<br /> <strong>Conclusions: </strong>Due to the increase of antibiotics resistance and the resistance to the pathogenic bacteria especially in intensive care units, it is necessary to find effective and accessible substances to destroy the resistant bacteria and reduce the mortality rate of patients. The results of the present study revealed that the antibacterial property of the shallot and the nettle alcoholic extracts could increase the antibacterial property of the silver nanoparticles. As a result, these can be used for disinfecting different wards of a hospital, in particular, the intensive care units. https://www.biotechrep.ir/article_100749_4680faf66bc397d64b4c6f483a145307.pdf