TY - JOUR ID - 68938 TI - Improvement of maize (Zea mays L.) anther culture embryogenesis and direct regeneration by differentplant growth regulators and gelling agent JO - Journal of Applied Biotechnology Reports JA - JABR LA - en SN - 2322-1186 AU - Hosseini, Seyedeh Zahra AU - Ismaili, Ahmad AU - Pour Mohammadi, Payam AD - Department of Plant Biology, Faculty of Basic Sciences, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran AD - Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khoram Abad, Iran AD - Department ofBiotechnology and Plant Breeding, Faculty of Agriculture, Ramin University of Agriculture and Natural Resources, Khouzestan, Iran Y1 - 2014 PY - 2014 VL - 1 IS - 1 SP - 17 EP - 21 KW - Anther culture,Directregeneration KW - Plantlet, Zea mays L DO - N2 - Androgenesis via anther culture or microspore culture is one of the current methods for producing haploid and double haploid plants in maize. To use of this method in maize breeding program it should be able to regenerate enough plantlets. Anthers culture usually is carried out indirectly via callus induction and regeneration on at least two different media. In this study a responsive genotype, ETH-M82, using a new single culture medium was used for embryogenesis and regeneration. We tested different growth regulators (2,4-D; kinetin; NAA & IAA) in modified YP medium. After6-week, direct regeneration on some treatments was observed. The highest frequency of direct formation of plantlets (in 100 anthers) occurred on medium supplemented with 2mgl-1 IAA and 2mgl-1 kinetin (4%).Best results with an average of 3.1plantlets in replication were obtained with the medium solidified with agar, while in difcobactoagar only 1.4 of plantlets in replication was produced. This experiment suggested that agar and plant growth regulators in the medium were beneficial for producing embryo and plantlet from maize anthers. UR - https://www.biotechrep.ir/article_68938.html L1 - https://www.biotechrep.ir/article_68938_93d974d94e4c54137d1cc651de82e035.pdf ER -