TY - JOUR ID - 106075 TI - Investigation the Effect of Silver Nanoparticles and Bioresonance Wave Radiation on Leishmania major: An In Vitro Study JO - Journal of Applied Biotechnology Reports JA - JABR LA - en SN - 2322-1186 AU - Azimijou, Nayereh AU - Keshvari, Hamid AU - Seyyed Tabaei, Seyyed Javad AU - Rahimi, Mohsen AU - Imanzadeh, Mehrdad AD - Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran AD - Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran AD - Department of Parasitology and Mycology, School of Medicine, Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran AD - Health Technology Research Institute, Amirkabir University of Technology, Tehran, Iran Y1 - 2020 PY - 2020 VL - 7 IS - 1 SP - 53 EP - 58 KW - Bioresonance KW - Leishmaniasis KW - Promastigote Stage KW - Silver nanoparticles DO - 10.30491/jabr.2020.106075 N2 - Introduction: Cutaneous leishmaniasis (CL) is one of the infectious diseases and health problems in tropical regions. Glucantime is commonly used to treat CL and it, not only has some side effects but also observation shows the drug resistance of some of the various Leishmania species. Therefore, the aim of this study was to investigate the effect of silver nanoparticles (AgNPs) and bioresonance waves on Leishmania, in vitro. Materials and Methods: In the present experimental study, Leishmania major promastigotes were cultured in RPMI-1640 supplemented with 10% FBS and 1% penicillin and streptomycin at 23°C. After 6 days, the parasites achieved stationary phases of promastigotes, Then the effects of different concentrations of AgNPs (1, 3, 5, 10 and 25 μg/mL) and different radiation times of bioresonance wave (5 and 20 minutes) were investigated. Herein, the effects of various treatment on parasites proliferation were evaluated with live promastigotes counting after 24, 48 and 72 hours treatment. Results: The parasite count showed that the various concentrations of AgNPs, radiation of bioresonance wave and combination significantly decreased the numbers of live promastigotes over time compared with the control group after 72 hours. The highest antileishmanial activity was seen for AgNPs at concentration of 1 μg/mL when combined with 20 minutes radiation of bioresonance wave (proliferation inhibition: 79.92%). Conclusions: Based on our result, AgNPs and bioresonance waves are potent antileishmanial agents. The authors declare that the more studies should be done. UR - https://www.biotechrep.ir/article_106075.html L1 - https://www.biotechrep.ir/article_106075_d891cd0aeeb864e30ce54dd8a86b4f98.pdf ER -