%0 Journal Article %T Determination of T-2 Mycotoxin in Fusarium strains by HPLC with fluorescence detector %J Journal of Applied Biotechnology Reports %I Baqiyatallah University of Medical Sciences %Z 2322-1186 %A Kachuei, Reza %A Rezaie, Sassan %A Yadegari, Mohammad Hossein %A Safaie, Naser %A Allameh, Abdol-Amir %A Aref-poor, Mohammad-Ali %A Imani Fooladi, Abbas-Ali %A Riazipour, Majid %D 2014 %\ 03/01/2014 %V 1 %N 1 %P 38-43 %! Determination of T-2 Mycotoxin in Fusarium strains by HPLC with fluorescence detector %K Fusarium %K Trichothecene %K T-2 toxin %K HPLC-F %R %X T-2 toxin is the most poisonous trichothecene produced by Fusarium species especially F.sporotrichioides. T-2 toxin is a biological contaminant in a number of agricultural commodities that can cause severe diseases among humans and animals and even lead to death. The aim of the current study is the analysis of T-2 mycotoxin in Fusarium species by high-performance liquid chromatography (HPLC) combined with florescence detection and derivatization with 1-antroylnitrile (1-AN). Totally, 11Fusariumisolates and reference strains were studied. The isolates were tested for the T-2 toxin production after growing on rice substrate followed by using specific “Multisep 225 Trich Clean up columns” purification. In this study, T-2 toxin production was ranged from 197.05ug/kg to 8503.07ug/kg. This is the first study of T-2 toxin analysis by HPLC-F in Iran. %U https://www.biotechrep.ir/article_68941_56d33ed14a114b64f7195cdcf1c68c27.pdf