Journal of Applied Biotechnology Reports

Journal of Applied Biotechnology Reports

A New and Efficient Method for DNA Extraction from Human Skeletal Remains Usable in DNA Typing

Document Type : Original Article

Authors
Ali Mohammadi 1
Akbar Ghorbani Alvanegh 1, 2
Mostafa Khafaei 1
Sajjad Habibi Azarian 1
Mahdi Naderi 1
Ebrahim Kiyani 1
Ali Miri 1
Hasan Bahmani 1
Maryam Ramezani 1
Mahmood Tavallaei 1
1 Human Genetics Research Center, Baqiyatallah University of Medical Sciences Tehran, Iran
2 Young Researchers and Elite Club, Islamic Azad University, Tehran, Iran
Abstract
In historical cases, mass disasters, missing person’s identification and ancient DNA investigations, bone and teeth samples are often the best and the only biological material available for DNA typing. This is because of the physical and chemical nature of the protein-mineral matrix of bone which is relatively resistant to the adverse environmental effects and biological attacks. Most bone extraction protocols used in the forensic laboratories involve an incubation period of bone powder in extraction buffer for proteinase digestion, followed by the collection of the supernatant, and the elimination of large quantities of undigested bone powder. Here we demonstrate an extremely effective protocol for recovery of DNA. This is performed in a method that retains and concentrates all the reagent volume for complete DNA recovery. For our study, we selected challenging bone samples of skeleton remains of the martyred individuals in Iraq’s imposed war on I.R. Iran (1980-1988). The bones that were extracted with our new protocol showed that this new protocol significantly enhances the quantity of DNA that can be used for amplification from degraded skeletal remains. At the same time we tested in parallel the samples by using of QIAamp DNA Investigator Kit and attained the best results by using new protocol. In fact, our new DNA extraction method is based on previous standard methods such as Chelex and salting out. We have used this technique to successfully recover authentic DNA Typing from extremely challenging samples that failed repeatedly using the standard protocols. However, the amount of recovered DNA was very small but it was possible to extract genomic DNA from these challenging bone samples. The results indicated that our procedure for DNA extraction although yielded little amount of genomic DNA; however, it was pure DNA that can be used for further analysis such as PCR amplification and DNA profiling. Since the new procedure is fast and needed less time than previously standard procedures, we have named it FDEB (Fast DNA Extraction of Bone).
Keywords
Bone
DNA Extraction
PCR
STR Profiling
Identification
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Journal of Applied Biotechnology Reports
Volume 4, Issue 2
Spring 2017
Pages 609-614

  • Receive Date 11 June 2017
  • Revise Date 11 July 2017
  • Accept Date 11 July 2017