Comparative Study of the Antioxidant, Antimicrobial and Anti-Inflammatory Activity between Essential Oil and Hydrosol Extract of the Aerial Parts of Inula viscosa L

Ainseba, Nabila; Soulimane, Amina; DIB, Mohammed El Amine; Djabou, Nassim; Muselli, Alain

doi:10.30491/jabr.2023.409230.1654

Comparative Study of the Antioxidant, Antimicrobial and Anti-Inflammatory Activity between Essential Oil and Hydrosol Extract of the Aerial Parts of Inula viscosa L

Document Type : Original Article

Authors

Nabila Ainseba ¹^{, 2}

Amina Soulimane ³

Mohammed El Amine DIB ⁴

Nassim Djabou ²

Alain Muselli ⁵

¹ Laboratoire de Chimie Organique, Substances Naturelles et Analyses, Universite Abou Bekr Belkaid Tlemcen, BP 119, 13000 Algérie

² Maghnia University Center, Tlemcen Algeria

³ Laboratoire d’Ecologie et Gestion des Ecosystèmes Naturels, Universite Abou Bekr Belkaid Tlemcen, BP 119, 13000; Algérie

⁴ Laboratoire des Substances Naturelles et Bioactives, Universite Abou Bekr Belkaid Tlemcen, BP 119, 13000; Algérie

⁵ Laboratoire Chimie des Produits Naturels, Université de Corse, UMR CNRS 6134, Campus Grimaldi, BP 52, FR-20250 Corté, France

10.30491/jabr.2023.409230.1654

Abstract

Introduction: The main objective of this study was to determine the chemical composition of the aerial part of the essential oil and the hydrosol extract of Inula viscosa which has never been studied and to compare their antioxidant, anti-inflammatory, and antimicrobial properties in vitro. This was done in order to identify new biologically active agents.
Materials and Methods: The essential oil and hydrosol extract were analyzed by GC and GC/MS. Antimicrobial activity was tested against three bacteria and two fungi. The antioxidant activities were assessed using three different methods: radical scavenging activity (DPPH), the β-carotene bleaching test, and Ferric-Reducing Antioxidant Power (FRAP). The anti-inflammatory activity was assessed using the protein denaturation method.
Results: The essential oil of Inula viscosa was composed mainly of hydrocarbon sesquiterpenes (52.6%) and oxygenated sesquiterpenes (47.0%), while the hydrosol extract was mainly composed of oxygenated sesquiterpenes (86.6%). The results of the biological activities showed that the hydrosol extract exhibited an interesting antioxidant activity, nearly equivalent to the synthetic antioxidant BHT. Furthermore, the hydrosol extract displayed very good anti-inflammatory activity, with an IC50 of 0.51 g/L, in comparison to diclofenac sodium (IC50 = 0.63 g/L). The hydrosol extract also exhibited antimicrobial activity and acted as an effective inhibitor of Bacillus cereus, Pseudomonas aeruginosa, Candida ATCC 26960, and Candida ATCC 10231 microorganisms.
Conclusions: The hydrosol extract of I. viscosa make this specie a potential alternative natural for use in the food and pharmaceutical industries. It can be utilized in the treatment of diseases involving oxidative stress as well as in the treatment of microbial and inflammatory infections.

Keywords

Hydrosol Extract

Essential Oil

Anti-inflammatory Activity

Antioxidant Activity

Antimicrobial Activity

Natural Products