Implementation of the Electrochemical Impedance Method for Measuring Troponin Enzyme in the Laboratory on a Chip

Sajedeh Jafarypour, Sajedeh; Rahdar, Mohebali; Farnoosh, Gholamreza

doi:10.30491/jabr.2022.333178.1506

Implementation of the Electrochemical Impedance Method for Measuring Troponin Enzyme in the Laboratory on a Chip

Document Type : Original Article

Authors

Sajedeh Sajedeh Jafarypour ¹

Mohebali Rahdar ¹

Gholamreza Farnoosh ²

¹ Department of Industrial Engineering, Faculty of Engineering, Sistan and Baluchestan University, Sistan and Baluchestan, Iran

² Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

10.30491/jabr.2022.333178.1506

Abstract

Introduction: Troponin enzyme is a gold biomarker for detecting heart attacks. IF it is possible to measure blood troponin levels at the onset of heart pain or symptoms of myocardial infarction, heart damage can be determined by measuring how much it changes. Diagnostic kits are available on the market to detect the presence of enzymes in the blood, but these kits do not check the extent of enzymatic changes. Actually, these kits only check for more than a certain amount of enzyme in the blood. Many experiments can be performed with the advent of lab-onchips.
Materials and Methods: In the proposed method, the troponin enzyme is separated from the rest of the blood by the selected aptamer and then the concentration of the troponin enzyme in the sample is measured by the electrochemical impedance method and Arduino board and coding. The Arduino software was used for coding and simulation, and electrochemical spectroscopy was performed by simulating the behavior of an electrochemical sensor.
Results: According to the findings of the present study, the initial measurement by the device showed an error of 55%, which was reduced to 13.5% in the measurement by changing the measuring factors.
Conclusions: The manufactured device has the ability to receive the sample, separate it into essential and non-essential components, extract the required information from the sample and also analyze the obtained information. However, the electronic structural factors of the device such as resistance, etc. but must be changed in order to reach 95% reliability.

Keywords

Troponin Enzyme

Lab On Chips

Enzyme Level Measurement

Myocardial infarction

Feasibility