An Overview of Culture Conditions for Recombinant Protein Expression in Escherichia coli

Maksum, Iman Permana; Latifah, Firdausi Permata Ummu; Nabiel, Ahmad; Sriwidodo, Sriwidodo; Lestari, Niky Fuzi; Azizah, Mamlikatu Ilmi; Utami, Diandra Firdiani

doi:10.30491/jabr.2022.349844.1547

An Overview of Culture Conditions for Recombinant Protein Expression in Escherichia coli

Document Type : Review Article

Authors

Iman Permana Maksum ¹

Firdausi Permata Ummu Latifah ¹

Ahmad Nabiel ¹

Sriwidodo Sriwidodo ²

Niky Fuzi Lestari ¹

Mamlikatu Ilmi Azizah ¹

Diandra Firdiani Utami ¹

¹ Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Sumedang, 45363, Indonesia

² Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, 45363, Indonesia

10.30491/jabr.2022.349844.1547

Abstract

Escherichia coli is one of the most commonly used organisms for producing recombinant protein. The recognized cell genome and well-known cell factory of the bacteria make it an ideal heterologous system of preference for the production of recombinant proteins. Over recent years, this cell system has been modified to improve the production of therapeutic proteins, and several methodologies are now available. One of the scientists' most preferred strategies is maintaining cell survival by optimizing the culture conditions. This review summarises some experiments related to those culture conditions and discusses how they affect recombinant protein expression in E. coli.

Keywords

Recombinant Protein

Escherichia coli

Culture Expression Parameters