Introduction:The last four decades have recognized the critical environmental issues pertaining to the use of non-degradable synthetic plastics. Currently, the synthesis of biodegradable polymers, like polyhydroxyalkaonoates (PHA) has been gaining considerable interest as a sustainable approach to overcome these issues. The current study was carried out with an objective to optimize the PHA production from Bacillus megateriumJHA. Materials and Methods: Various nutritional and physico-chemical parameters were optimized using the ‘one factor at a time’ approach. The findings were analysed statistically by one-way ANNOVA and linear regression using the open-source R software. Results:The optimum physico-chemical parameters for maximum PHA production were attained when 5% inoculum size of B. megateriumJHA was added to the JHA medium (pH 8) and incubated at 28 °C for 96 h under shaker conditions (120 rpm). The nutritional parameters were further optimized by addition of 0.6 g% K2HPO4, 0.4 g% KH2PO4, 21 g% glucose, 12.5 mM microcosmic salt, 0.04 g% KNO3, 1 mM MgSO4, and 2 ml trace elements to the JHA medium. The C:N and C:P ratio was adjusted to 70:1 and 20:1 respectively. The growth of bacteria under these optimized parameters allowed 13.71 g/L accumulation of PHA leading to a yield of 54.51%. This resulted in 66.88% increase in yield as compared to the original medium. The scale-up study using a 2 L fermenter further increased PHA accumulation by 4.39% under optimized conditions. Conclusions:The above results clearly indicate that B. megateriumJHA is a promising isolate that can be exploited for the industrial production of PHA.
Mascarenhas,J and K,A . (2021). Production and Optimization of Polyhydroxyalkaonoate Obtained from Bacillus megaterium JHA. Journal of Applied Biotechnology Reports, 8(4), 346-360. doi: 10.30491/jabr.2020.242493.1263
MLA
Mascarenhas,J , and K,A . "Production and Optimization of Polyhydroxyalkaonoate Obtained from Bacillus megaterium JHA", Journal of Applied Biotechnology Reports, 8, 4, 2021, 346-360. doi: 10.30491/jabr.2020.242493.1263
HARVARD
Mascarenhas J, K A. (2021). 'Production and Optimization of Polyhydroxyalkaonoate Obtained from Bacillus megaterium JHA', Journal of Applied Biotechnology Reports, 8(4), pp. 346-360. doi: 10.30491/jabr.2020.242493.1263
CHICAGO
J Mascarenhas and A K, "Production and Optimization of Polyhydroxyalkaonoate Obtained from Bacillus megaterium JHA," Journal of Applied Biotechnology Reports, 8 4 (2021): 346-360, doi: 10.30491/jabr.2020.242493.1263
VANCOUVER
Mascarenhas J, K A. Production and Optimization of Polyhydroxyalkaonoate Obtained from Bacillus megaterium JHA. J Apple Biotechnol Rep. 2021;8(4):346-360. doi: 10.30491/jabr.2020.242493.1263
