An Effective Method for Extracting DNA From Human Hair Samples

Khafaei, Mostafa; Sadeghi, Morteza; Zargari, Peyman; Saadatmand, Fatemeh; Barouei, Roghayeh; Mohammadi, Ali

doi:10.30491/jabr.2020.117890

An Effective Method for Extracting DNA From Human Hair Samples

Document Type : Original Article

Authors

Mostafa Khafaei ¹

Morteza Sadeghi ¹

Peyman Zargari ¹

Fatemeh Saadatmand ¹

Roghayeh Barouei ²

Ali Mohammadi ¹^{, 3}

¹ Human Genetics Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

² Department of Biology, Payame Noor University, Mashhad, Iran

³ Molecular Detection Laboratory, Valiasr Medical Clinic, Mashhad, Iran

10.30491/jabr.2020.117890

Abstract

Introduction: In historical cases, mass disasters, missing person’s identification, and Archaeogenetic investigations, the bone, teeth, and hair samples are often the best and the only biological material available for DNA typing. In this study, we demonstrated an extremely effective protocol for DNA extraction of hair.
Materials and Methods: Obtaining genetic information from hair samples for DNA typing involves the following steps: sterilization, DNA extraction, quality control, PCR, and profiling. After extracting aDNA (Ancient DNA) from the hair, autosomal STR analyses were performed for each sample using the AmpFℓSTR® MiniFiler™ and Identifiler™ kits.
Result: Qualitative analysis of DNA by electrophoresis showed the excellent quality of the extracted samples so that the electrophoresed bands were clearly visible.
Conclusions: The results demonstrated that the FDEH method is cheaper and faster than commercial kits; moreover, this new method enhances DNA recovery from hair. Because of simple protocol and high quality, this method can be utilized in Medical.

Keywords

Hair

DNA extraction

PCR

DNA Profiling

Identification