A Review of the Methods for Concentrating M13 Phage

Fouladvand, Faezeh; Bemani, Peyman; Mohammadi, Mozafar; Amini, Razieh; Azizi Jalilian, Farid

doi:10.30491/jabr.2020.105916

A Review of the Methods for Concentrating M13 Phage

Document Type : Review Article

Authors

Faezeh Fouladvand ¹

Peyman Bemani ²

Mozafar Mohammadi ³

Razieh Amini ¹

Farid Azizi Jalilian ⁴

¹ Department of Molecular Medicine and Genetic, Faculty of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran

² Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran

³ Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

⁴ Department of Virology, Faculty of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran

10.30491/jabr.2020.105916

Abstract

Bacteriophages, viruses which infect the bacteria are the most abundant organisms on the earth. Among them, the best studied and the most-exploited group is the filamentous phages especially M13 phage. They have shown a lot of interesting applications because of their unique features. Therefore, to get maximum performance of bacteriophage like M13, techniques need to be engaged for proper concentrations. In this review, most of these methods were explored in PubMed, Scopus and Google Scholar, using keywords including M13 bacteriophage, phage concentration, phage purification, phage display. Accordingly, the most important research papers about this subject have been collected, categorized and discussed. As a conclusion, to select an appropriate method for the concentration of M13 bacteriophages different criteria should be considered, including cost, equipment, yield and purity of the product. In general, subsequent applications of M13 phage is the most important factor for the selection of the concentration method.

Keywords

M13 Bacteriophage

Phage Concentration

Phage Purification

Phage Display

Phage Precipitation