Document Type: Original Article
Human Genetics Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Human Genetics Research Center, Baqiyatallah University of Medical Sciences Tehran, Iran
Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
Introduction: Recent studies have indicated the function of polymorphisms in the miRNAs-binding sites within the target genes in cancer incidence. The aim of this study was to investigate the relationship between KRAS rs712 polymorphism within let-7 microRNA-binding site and lung cancer (LC) risk in the Iranian population.
Materials and Methods: This case-control study was performed among 100 patients with LC and 100 healthy individuals. The rs712 polymorphism in KRAS gene promoter was evaluated using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique and direct sequencing. Statistical analysis was performed using SPSS software and t-test.
Results: The findings of this study revealed that rs712, TT and GT genotypes are significantly associated with LC incidence in the Iranian population (adjusted odds ratio [OR] = 3.556, 95% CI = 1.346–9.379, P = 0.01) and (OR = 2.45, 95% CI = 1.274–4.719, P = 0.01), and rs712 T allele was associated with LC incidence (OR = 1.711, 95% CI = 1.147–2.553, P = 0.01). In addition, our analysis showed an association between the rs712 TT genotype and LC metastasis (P = 0.006).
Conclusions: According to our findings, rs712 T allele is significantly associated with the initiation and progression of LC in the Iranian population and it may be served as a LC screening marker in the future.