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Abdollah, Z., Khodi, S., Gheybi, E., Amani, J., Karami, A. (2018). Recombinant Expression and Purification of Human Cystatin C Biomarker in Escherichia coli for Prediagnostic Renal Disorders. Journal of Applied Biotechnology Reports, 5(1), 13-18.
Zahra Abdollah; Samaneh Khodi; Elaheh Gheybi; Jafar Amani; Ali Karami. "Recombinant Expression and Purification of Human Cystatin C Biomarker in Escherichia coli for Prediagnostic Renal Disorders". Journal of Applied Biotechnology Reports, 5, 1, 2018, 13-18.
Abdollah, Z., Khodi, S., Gheybi, E., Amani, J., Karami, A. (2018). 'Recombinant Expression and Purification of Human Cystatin C Biomarker in Escherichia coli for Prediagnostic Renal Disorders', Journal of Applied Biotechnology Reports, 5(1), pp. 13-18.
Abdollah, Z., Khodi, S., Gheybi, E., Amani, J., Karami, A. Recombinant Expression and Purification of Human Cystatin C Biomarker in Escherichia coli for Prediagnostic Renal Disorders. Journal of Applied Biotechnology Reports, 2018; 5(1): 13-18.

Recombinant Expression and Purification of Human Cystatin C Biomarker in Escherichia coli for Prediagnostic Renal Disorders

Article 3, Volume 5, Issue 1, Winter 2018, Page 13-18  XML PDF (373.07 K)
Document Type: Original Article
Authors
Zahra Abdollah1; Samaneh Khodi1; Elaheh Gheybi2; Jafar Amani email 3; Ali Karami email 4
1Applied Biotechnology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
2Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran
3Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
4Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
Receive Date: 11 November 2017,  Revise Date: 03 February 2018,  Accept Date: 15 February 2018 
Abstract
Introduction: Chronic kidney disease is a worldwide health problem and Glomerular filtration rate (GFR) is the most frequently used criteria in the assessment of rental function. Cystatin C as a member of type 2 cystatin superfamily and cysteine-protease inhibitor is found in high concentrations in all biological fluids. This study is aimed to study cystatin C as a potent biomarker for clinical measurement of renal disorders and other diseases.
Materials and Methods: In this study, the human cystatin C construct was analyzed by bioinformatics software. It was cloned and expressed to produce an appropriate antigen for anti-cystatin C (anti-Cys C) obtained from mice Balb/C as a crucial point in the improvement of an enzyme-linked immunosorbent assay (ELISA) method. Serum samples were given from 32 hospitalized patients with renal failure and cardiovascular disease and non-hospitalized patients were tested by ELISA method using anti-Cys C obtained from mice Balb/C.
Results: Our findings indicated 0.36-2.4 mg/L as the best conclusion for antigen cystatin C in patients’ sera and 1/50 dilution for anti-Cys C obtained from mice Balb/C and showed a relationship between patients with high creatinine and high concentration of cystatin C. In case of five cardiovascular disease patients with normal upper limit of Creatinine we obtained cystatin C lower than kidney failure and raising of cystatin C in 6 patients with increased TSH were seen.
Conclusions: Polyclonal anti-Cys C antibodies were obtained through the immunization of Balb/C mice can be employed as an anti-Cys C in ELISA for diagnosis of some renal dysfunction.
Keywords
Creatinine; Cystatin C; Enzyme-Linked Immunosorbent Assay (ELISA); Glomerular Filtration Rate (GFR)
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