1
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2
Department of Chemistry, Islamic Azad University, Lahijan Branch, Lahijan, Iran
Abstract
Calamintha officinalis (COM) is a genus of aromatic herbs, native in north of Iran. Partial purification of aqueous extract from COM was done using ammonium sulfate precipitation followed by dialysis. The total protein content was determined at various purification stages. The highest amount of protein of the extract was found in fraction obtained in 20-40% saturation range. Specific activities of three antioxidant enzymes, peroxidase, catalase, and superoxide dismutase were also determined and compared in each stage. The highest activity of peroxidase, catalase and superoxide dismutase was in fractions 60-80%, 20-40%, and 20-40% respectively. The non-enzymatic antioxidants were evaluated by two different methods i.e. free radical scavenging activity using DPPH and inhibition of lipid peroxidation by the ferric thiocyanate method. It was found that radical scavenging power of COM methanolic extract was 43.99. Furthermore, the inhibition of li pid peroxidation of the extract was decreased every 24 h. Thus, our findings suggested that C. officinalis possess potent antioxidant defense machinery both enzymatic and non enzymatic.
Racchi, M.L., Antioxidant defenses in plants with attention to Prunus and Citrus spp. Antioxidants, 2013, Vol. 2, pp. 340-369.
Kamalanathan, D., Srinivasan, R., Pratheeba, T., Yuvarajan, R., Natarajan, D., Antioxidant activities of leaf extracts of Euphorbia fusiformis Buch.-Ham. ex D. Don (Euphorbiaceae). Free RadicAntioxdant, 2015, Vol. 5, pp. 83-89.
Duman, Y.A., Kaya, E., Three-Phase partitioning as a rapid and easy method for the purification and recovery of catalase from sweet potato tubers (Solanum tuberosum). Appl Biochem Biotechnol, 2013, Vol. 170, pp. 1119-1126.
Sharma, A.D., Rakhra, G., Kaur, S., Kaur, H., Expression of boiling-stable peroxidase (PRX) isoenzymes under combined effect of drought and heat in different tissues of Triticum aestivum. J Agric Technol, 2013, Vol. 9, pp. 901-913.
Niyomploy, P., Boonsombat, R., Karnchanatat, A., Sangvanich, P., A superoxide dismutase purified from the roots from Stemona tuberosa. Prep Biochem Biotechnol, 2014a, Vol. 44, pp. 663-679.
Niyomploy, P., Srisomsap, C., Chokchaichamnankit, D., Vinayavekhin, N., Karnchanatat, A., Sangvanich, P., Superoxide dismutase isozyme detection using two-dimensional gel electrophoresis zymograms. J Pharm Biomel Anal, 2014b, Vol. 90, pp. 72-77.
Lai, L.S., Chang, P.C., Chang, C.T., Isolation and characterization of superoxide dismutase from wheat seedlings. J Agric Food Chem, 2008, Vol. 56, pp. 8121-9.
Philippe, B.A., Karine, N., Barthélemy, A.K., Noél, Z.G., Joseph, D.A., Hosttetmann, K., Bio-guided isolation of antioxidant compounds from Chrysophyllum perpulchrum, a plant used in the Ivory Coast pharmacopeia. Molecules, 2010, Vol. 15, pp. 6386-6398.
Tomsone, L., Kruma, Z., Galoburda, R., Comparison of different solvents and extraction methods for isolation of phenolic compounds from horseradish roots (Armoracia rusticana). World Acad Sci Eng Technol, 2012, Vol. 64, pp. 903-908.
Alam, M.N., Bristi, N.J., Rafiquzzaman, M., Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharm J, 2013, Vol. 21, pp. 143-52.
Koksal, E., Gulcin, I., Purification and characterization of peroxidase from cauliflower (Brassica oleracea L. var. botrytis) buds. Protein Pep Lett, 2008, Vol. 15, pp. 320-326.
Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 1976, Vol. 72, pp. 248-24.
Rached-Kanouni, M., Alatou, D., Change in activity of antioxidative enzymes in leaves of Acacia retinodes, Biota orientalis and Casuarina equisetifolia under heat stress condition. Eur Sc J, 2013, Vol. 9, pp. 402-410.
VG, M.K., Murugan, K., Antioxidant potentiality of partially purified protease inhibitor from the fruits of African nightshade (Solanum aculeatissimum Jacq.). World J Pharm Pharm Sci, 2013, Vol. 2, pp. 5166-5181.
Sisecioglu, M., Atasever, A., Şehitoğlu, M.H., Kaya, H.B., Purification and characterization of peroxidase from Turkish black radish (Raphanus sativus L.). J Med Plants Res, 2010, Vol. 4, pp. 1187-1196.
Hamid, M., Majeed, H., Al-Assie A.H., Purification and characterization of peroxidase extracted from wheat bran. World J Pharm Res, 2016, Vol. 5, pp. 77-86.
B en Khaled, H., Ktari, N., Siala, R., Hmidet, N., Bayoudh, A., Nasri, M., Ghorbel, S., A heat-stable Cu/Zn superoxide dismutase from the viscera of sardinelle (Sardinella aurita): purification and biochemical characterization. Biologia, 2014, Vol. 69, pp. 1770-1776.
Mohammadi-Motamed, S., Shahidi-Motlagh, S., Bagherzadeh, H., Azad Forouz, S., Tafazoli, H., Evaluation of antioxidant activity of Ruta graveolens L. extract on inhibition of lipid peroxidation and DPPH radicals and the effects of some external factors on plant extract's potency. Res J Pharmacogn, 2014, Vol. 1, pp. 45-50.
Shams Moattar, F., Sariri, R., Yaghmaee, P., & Giahi, M. (2016). Enzymatic and Non-enzymatic Antioxidants of Calamintha officinalis Moench Extracts. Journal of Applied Biotechnology Reports, 3(4), 489-494.
MLA
Fatemeh Shams Moattar; Reyhaneh Sariri; Parichehr Yaghmaee; Masoud Giahi. "Enzymatic and Non-enzymatic Antioxidants of Calamintha officinalis Moench Extracts". Journal of Applied Biotechnology Reports, 3, 4, 2016, 489-494.
HARVARD
Shams Moattar, F., Sariri, R., Yaghmaee, P., Giahi, M. (2016). 'Enzymatic and Non-enzymatic Antioxidants of Calamintha officinalis Moench Extracts', Journal of Applied Biotechnology Reports, 3(4), pp. 489-494.
VANCOUVER
Shams Moattar, F., Sariri, R., Yaghmaee, P., Giahi, M. Enzymatic and Non-enzymatic Antioxidants of Calamintha officinalis Moench Extracts. Journal of Applied Biotechnology Reports, 2016; 3(4): 489-494.
)