Biochemical and Physiological Characterization of Tree Microalgae spp. as Candidates for Food Supplement

Document Type: Original Article

Authors

1 Department of Petroleum Microbiology, Research Institute of Applied Science, ACECR, Tehran, Iran

2 Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

3 Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran

4 Department of Biology, Gorgan Branch, Islamic Azad University, Gorgan, Iran

5 Department of Biology, Payamnoor University, Tehran, Iran

Abstract

With increasing world populations, production of cost-effective and proper nourishment sources that can rapidly produce large amounts of nutritional value are needed. Microalgae are publicly used as nutrient supplement. In this research a screening of endemic potent microalgae was carried out. Chlorella, Scenedesmus and Spirulina sp. were isolated and purified and cultivated in liquid proper medium. Regarding to this, amino acid and fatty acid profiles, biochemical characters, antioxidant and antimicrobial and anticancer properties of experimented microalgae were evaluated by HPLC, GC, spectrophotometry, DPPH, MIC and MTT Assay respectively. The results showed highest content of total protein in Spirulina sp.1 (46.08 ppm) and total carbohydrates in Chlorella sp. (48.01 ppm). Antioxidant content was detected in mentioned microalgae. Cytotoxic effect of aqueousextract on L929 cells showed 10 mg/mL had highest effect on these cells. According to the results, Chlorella spp. and Spirulina spp.1 are better candidates for food
supplement.

Keywords


  1. Shay, E.G., Diesel fuel from vegetable oils: status and opportunities. Biomass Bioenerg, 1993, Vol. 4, pp. 227-242.
  2. 2. Richmond, A., Handbook of microalgal mass culture. Vol. 528. 1986: CRC press Boca Raton, FL.
  3. 3. Gao, K., Chinese studies on the edible blue-green alga, Nostoc flagelliforme: a review. J Appl Phycol, 1998, Vol. 10, pp. 37-49.
  4. 4. Pulz, O., Gross, W., Valuable products from biotechnology of microalgae. Appl Microbiol Biotechnol, 2004, Vol. 65, pp. 635-648.
  5. 5. West, M., Evaluation of microalgae for use as nutraceuticals and nutritional supplements. J Nutr Food Sci, 2012, Vol. 2, pp. 147-152.
  6. 6. Yamaguchi, K., Recent advances in microalgal bioscience in Japan, with special reference to utilization of biomass and metabolites: a review. J Appl Phycol, 1996, Vol. 8, pp. 487-502.
  7. 7. Spolaore, P., Joannis-Cassan, C., Duran, E., Isambert, A., Commercial applications of microalgae. J Biosci Bioeng, 2006, Vol. 101, pp. 87-96.
  8. 8. Ötle , S., Pire, R.e., Fatty acid composition of Chlorella and Spirulina microalgae species. J AOAC Int, 2001, Vol. 84, pp. 1708-1714.
  9. 9. Shimamatsu, H., Mass production of Spirulina, an edible microalga, in Asian Pacific Phycology in the 21st Century: Prospects and Challenges. 2004, Springer. p. 39-44.
  10. 10. Reinehr, C.O., Costa, J.A.V., Repeated batch cultivation of the microalga Spirulina platensis. World J Microbiol Biotechnol, 2006, Vol. 22, pp. 937-943.
  11. 11. Kato, T., Blue pigment from Spirulina. New Food Indust, 1994, Vol. 29, pp. 17-21.12. Andersen, R.A., Algal culturing techniques. 2005: Academic press.
  12. 13. Soltani, N., Baftechi, L., Dezfulian, M., Shokravi, S., Alnajar, N., Molecular and morphological characterization of oil polluted microalgae. Int J Environ Res, 2012, Vol. 6, pp. 481-492.
  13. 14. Federation, W.E., Association, A.P.H., Standard methods for the examination of water and wastewater. American Public Health Association (APHA): Washington, DC, USA, 2005, Vol. pp.
  14. 15. Guan, W., Zhao, H., Lu, X., Wang, C., Yang, M., Bai, F., Quantitative analysis of fatty-acid-based biofuels produced by wild-type and genetically engineered cyanobacteria by gas chromatography–mass spectrometry. Journal Chromatogr, 2011, Vol. 1218, pp. 8289-8293.
  15. 16. Soletto, D., Binaghi, L., Lodi, A., Carvalho, J., Converti, A., Batch and fed-batch cultivations of Spirulina platensis using ammonium sulphate and urea as nitrogen sources. Aquaculture, 2005, Vol. 243, pp. 217-224.
  16. 17. Guil-Guerrero, J., Navarro-Juárez, R., López-Mart nez, J., Campra-Madrid, P., Rebolloso-Fuentes, M., Functional properties of the biomass of three microalgal species. J Food Eng, 2004, Vol. 65, pp. 511-517.
  17. 18. MA, B., Vitamins and fine chemicals from Microalgae, 1988, Borowitzka, MA and Borowitzka, LJ, Cambridge University Press.
  18. 19. Badarpa, N.M., Pereira, P.A., Batista, I., Vilela, M.H., fatty acids, sterols and tocopherol in isochrysis galbana. J Food Lip, 2003, Vol. 10, pp. 25-34.
  19. 20. Donato, M., Vilela, M.H., Bandarra, N.M., Fatty acids, Strols, Tocopherol and Total Carotenoids Composition of Diacronema Vlkianum. J Food Lip, 2003, Vol. 10, pp. 267-276.
  20. . Luiten, E.E., Akkerman, I., Koulman, A., Kamermans, P., Reith, H., Barbosa, M.J., Sipkema, D., Wijffels, R.H., Realizing the promises of marine biotechnology. Biomol Eng, 2003, Vol. 20, pp. 429-439.
  21. Natrah, F., Yusoff, F., Shariff, M., Abas, F., Mariana, N., Screening of Malaysian indigenous microalgae for antioxidant properties and nutritional value. J Appl Phycol, 2007, Vol. 19, pp. 711-718.
  22. Fedorov, S.N., Ermakova, S.P., Zvyagintseva, T.N., Stonik, V.A., Anticancer and cancer preventive properties of marine polysaccharides: Some results and prospects. Marine drugs, 2013, Vol. 11, pp. 4876-4901.
  23. Santoyo, S., Jaime, L., Plaza, M., Herrero, M., Rodriguez-Meizoso, I., Ibañez, E., Reglero, G., Antiviral compounds obtained from microalgae commonly used as carotenoid sources. J Appl Phycol, 2012, Vol. 24, pp. 731-741.
  24. Guedes, É.A., Silva, T.G.d., Aguiar, J.S., Barros, L.D.d., Pinotti, L.M., Sant'Ana, A.E., Cytotoxic activity of marine algae against cancerous cells. Revista Brasileira de Farmacognosia, 2013, Vol. 23, pp. 668-673.
  25. Papadopoulos, K.N., Food chemistry research developments. 2008: Nova Publishers.
  26. Beheshtipour, H., Mortazavian, A., Mohammadi, R., Sohrabvandi, S., Khosravi Darani, K., Supplementation of Spirulina platensis and Chlorella vulgaris algae into probiotic fermented milks. Compr Rev Food Sci Food Saf, 2013, Vol. 12, pp. 144-154.