Electrochemical Determination of Calcium Dobesilate on Graphene Glassy Carbon Modified Electrode

Document Type : Original Article

Authors

1 Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

2 Institute of Biochemistry & Biophysics, University of Tehran, Iran

3 Department of Energy Engineering and Physics, Faculty of Physics, Amirkabir University of Technology, Tehran, Iran

4 Nanobiotechnology Research Center, Baqiyatallah University of Medical Science, Tehran, Iran

Abstract

Graphene modified glassy carbon electrodes have been developed for the determination of calcium dobesilate in pharmaceutical formulations. The prepared graphene and CNTs were used as a conductive substrate for electrochemical study of calcium dobesilate. The direct electrochemistry of calcium dobesilate showed a reversible cyclic voltammogram with a formal potential of 133 mV (vs. Ag/AgCl) for graphene modified electrodes in 0.1 M phosphate buffer. The linear concentration range of the sensor is 30-120 ng/mL for graphene modified electrode, respectively. The lifetime of biosensor is more than 2 weeks. The proposed modified electrode provides a new promising and alternative way to detect calcium dobesilate

Keywords

References

Xin-Yuan Z., Wei1 L., Shan W., Jing-Long J., Wei-Hong L., Ning-Li W. Calcium dobesilate for diabetic retinopathy: a systematic review and meta-analysis. Sci China Life Sci. Vol. 58, pp. 101-107.
The United States Pharmacopoeia, USP 30, The National Formulary, 25th ed., United States Pharmacopeial Convention, Rockville, MD, 2007.
British Pharmacopoeia, The Stationery Office on Be-half of the Medicines andHealthcare Products Regulatory Agency (MHRA)- Crown Copyright, 2013.
Song J.F., Chen J.Q., Flow-injection bi-amperometric direct determination of calcium dobesilate in irreversible couple system. J Pharm Biomed Anal. 2003, Vol. 33, pp. 789-798.
Chen Y., Xiao Y., Detection of calcium dobesilate in calcium dobesilate capsules: a comparison of HPLC and UV spectrophotometry. 2008, Vol. 24, pp. 480-483.
Song Z., Yue Q., Wang C., Flow injection chemiluminescence fordetecting picogram amounts of dobesilate in human urine. Spectrochim Acta A. 2004, Vol. 10, pp. 2377-82.
Song Z., Zhang N., Wang L. In vitro determination of dobesilate inmedicine and human urine with chemiluminescence detection. Microchim Acta, 2003, Vol. 142, pp. 205-511.
Róna K., Ary K., Determination of Calcium dobesilate in human plasma using ion-pairing extraction and high-performance liquid chromatography. J Chromatogr B, 2001, Vol. 755, pp. 245-51.
K. Eskandari, Farideh Gouranlou, Mehdi Kamali, Ali Morsali, Int J Biomed Nanosci Nanotechnol, 2014, Vol. 3, pp. 85-88.
Wang S., Xu Q., Zhang X,. Liu G., Sensitive electrochemical determination of calcium dobesilate on the carbon–iron nanoparticle modified glassy carbon electrode. Electrochemistry Communications 2008, Vol. 10, pp. 411–415.
Journal of Applied Biotechnology Reports
Volume 3, Issue 1
Winter 2016
Pages 373-375

Files

History

  • Receive Date: 12 January 2017
  • Revise Date: 12 February 2017
  • Accept Date: 12 February 2017

Share

How to cite

Statistics