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Chehari, K., Sepahvand, F., Ghobadi, S., Ismaili, A., Rafiei Alavy, E. (2014). Study of Paraoxonase -1 Gene Polymorphism in a Healthy Population of Khorramabad, Iran. Journal of Applied Biotechnology Reports, 1(2), 81-85.
Kobra Chehari; Farideh Sepahvand; Sirous Ghobadi; Ahmad Ismaili; Ezat Rafiei Alavy. "Study of Paraoxonase -1 Gene Polymorphism in a Healthy Population of Khorramabad, Iran". Journal of Applied Biotechnology Reports, 1, 2, 2014, 81-85.
Chehari, K., Sepahvand, F., Ghobadi, S., Ismaili, A., Rafiei Alavy, E. (2014). 'Study of Paraoxonase -1 Gene Polymorphism in a Healthy Population of Khorramabad, Iran', Journal of Applied Biotechnology Reports, 1(2), pp. 81-85.
Chehari, K., Sepahvand, F., Ghobadi, S., Ismaili, A., Rafiei Alavy, E. Study of Paraoxonase -1 Gene Polymorphism in a Healthy Population of Khorramabad, Iran. Journal of Applied Biotechnology Reports, 2014; 1(2): 81-85.

Study of Paraoxonase -1 Gene Polymorphism in a Healthy Population of Khorramabad, Iran

Article 8, Volume 1, Issue 2, Spring 2014, Page 81-85  XML PDF (110.08 K)
Document Type: Original Article
Authors
Kobra Chehari1; Farideh Sepahvand2; Sirous Ghobadi1; Ahmad Ismaili email 3; Ezat Rafiei Alavy4
1Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran
2Department of Biology, Faculty of Science, Lorestan University, Khorrtamabad, Iran
3Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad, Iran
4Department of Pathology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
Receive Date: 25 March 2014,  Revise Date: 13 May 2014,  Accept Date: 13 May 2014 
Abstract
Human serum paraoxonase (HuPON1: EC 3.1.8.1), a calcium-dependent esterase, is synthesized in the liver and widely distributed in tissues including liver, kidney, intestine, and serum, where it is associated exclusively with high-density lipoprotein.  Human paraoxonase-1 plays an important role in prevention of atherosclerosis and also protection against organophosphate-induced neurotoxicity. Paraoxonase-1 shows 2 common polymorphisms: Q/R at position 192 and M/L at position 55. In this study, paraoxonase-1 192 and 55 polymorphisms were investigated in 64 healthy Iranian individuals. Genomic DNA was isolated from whole blood by the Bartlett method, and paraoxonase-1 genotypes were determined by polymerase chain reaction amplification followed by restriction isotyping and gel electrophoresis. The chi-square test was used to evaluate the Hardy-Weinberg equilibrium. The genotype frequencies for paraoxonase 1-Q192R­ were pproximately 47% (QQ), 41% (QR) and 12% (RR) and for paraoxonase-1 M55L, 44% (LL), 44% (ML) and 12% (MM). Thus, the frequency of alleles R, L, Q, and M were 0.33, 0.66, 0.67, and 0.34 respectively. In conclusion, the frequencies of paraoxonase-1 192 and 55 polymorphisms in this group of Iranian population were different from those seen in other Asian populations from Japan and China but similar to European (Caucasians).
Keywords
Atherosclerosis; Haplotype; Paraoxonase; Polymorphism; Polymerase Chain Reaction
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