Document Type: Original Article
Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran
Department of Biology, Faculty of Science, Lorestan University, Khorrtamabad, Iran
Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad, Iran
Department of Pathology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
Human serum paraoxonase (HuPON1: EC 188.8.131.52), a calcium-dependent esterase, is synthesized in the liver and widely distributed in tissues including liver, kidney, intestine, and serum, where it is associated exclusively with high-density lipoprotein. Human paraoxonase-1 plays an important role in prevention of atherosclerosis and also protection against organophosphate-induced neurotoxicity. Paraoxonase-1 shows 2 common polymorphisms: Q/R at position 192 and M/L at position 55. In this study, paraoxonase-1 192 and 55 polymorphisms were investigated in 64 healthy Iranian individuals. Genomic DNA was isolated from whole blood by the Bartlett method, and paraoxonase-1 genotypes were determined by polymerase chain reaction amplification followed by restriction isotyping and gel electrophoresis. The chi-square test was used to evaluate the Hardy-Weinberg equilibrium. The genotype frequencies for paraoxonase 1-Q192R were pproximately 47% (QQ), 41% (QR) and 12% (RR) and for paraoxonase-1 M55L, 44% (LL), 44% (ML) and 12% (MM). Thus, the frequency of alleles R, L, Q, and M were 0.33, 0.66, 0.67, and 0.34 respectively. In conclusion, the frequencies of paraoxonase-1 192 and 55 polymorphisms in this group of Iranian population were different from those seen in other Asian populations from Japan and China but similar to European (Caucasians).