The Inhibition Effects of Two Species of Astragalus Extracts on Mushroom Tyrosinase Activity

Document Type : Original Article

Authors

1 Department of Biology, School of Science and Agriculture, Payame Noor University of Tehran, Tehran, Iran

2 Department of Medicinal Chemistry, School of Pharmacy, Ahwaz Jundishapur University of Medical Sciences, Ahwaz, Iran

3 Department of Pharmacognosy, School of Pharmacy, Ahwaz Jundishapur University of Medical Sciences, Ahwaz, Iran

Abstract

Melanin pigment plays a critical role in camouflage and protection against harmful effects of solar radiation. Melanogenesis is under complex regulatory control by multiple agents. Tyrosinase is a multifunctional, which catalyzes the first two steps in mammalian melanogenesis. In this study, inhibitory effects of Astragalus fasciculifolius and Astragalus gypsicolus on diphenolase activity of mushroom tyrosinase were evaluated. .Ethanol 80% extracts were prepared for screening tests. The IC50, Ki, Km and Vm values of Astragalus fasciculifolius and Astragalus gypsicolus were measured then compared them. We found that Both extracts show mixed type inhibition on mushroom tyrosinase when L-DOPA was used as a substrate.

Keywords

References

  1. Kang, S., Kim, H., Jin, C., Lee, Y., Synthesis of tyrosinase inhibitory (4-oxo-4H-pyran-2-yl)acrylic acid ester derivatives. Bioorg Med ChemLett, 2009,Vol.19,pp. 188–191.
  2. Sapkota, K., Roh, E., Lee, E., Ha, E., Yang, J., Lee, E., Kwon, Y., Kim, Y., Na, Y., Synthesis and anti-melanogenic activity of hydroxyphenyl benzyl ether Analogues. Bioorg MedChem, 2011, Vol. 19, pp. 2168–2175.
  3. Nyonella, F., Marcella, C., Benedetta, E., M.Benedetta, F., Matos, M., Quezada, E., Santana, L., Piccaiau, C., Tyrosinase inhibitor activity of coumarin-resveratrol hybrids. Molecules, 2009, Vol. 14, pp. 2514-2520.
  4. Okombi, S., Rival, D., Bonnet, S., Mariotte, A., Perrierb, E and Boumendjel., A., Analogues of N-hydroxycinnamoylphenalkylamides as inhibitors of human melanocyte-tyrosinase. Bioorg Med ChemLett, 2006, Vol.16, pp. 2252–2255.
  5. Saboury, A., Alijanianzadeh, M and Mansoori-Torshizi, H., The role of alkyl chain length in the inhibitory effect n-alkyl xanthates on mushroom tyrosinase activities.Actabiochim pol, 2007, Vol. 54, pp. 183–192.
  6. Mitsuhiro, S., Yumi, W and Kazuo, U., Synthesis and tyrosinase inhibitory activity of novel N-hydroxybenzyl-N-nitrosohydroxylamines.BioorgChem, 2003, Vol. 31, pp. 129–135.
  7. Te, SC., An updated review of tyrosinaseinhiboitors. Int J MolSci, 2009, Vol.10, pp. 2440-2475.
  8. Kim, Y-J., Uyama, H., Tyrosinase inhibitors from natural and synthetic sources: structure, inhibition mechanism and perspective for the future.Cell Mollife Sci, 2005, Vol.62, pp.1707-1723.
  9. Lee, Y., Park, J., Kim, M., Seo, S., Kim, H., Synthesis of Tyrosinase Inhibitory Kojic Acid Derivative. Arch Pharm Chem Life Sci, 2006, Vol. 339,pp.111–114.
  10. Zheng, ZP., Cheng, KW., To, JT., Li, H., Wang, M.,Isolation of tyrosinase inhibitors from Artocarpusheterophyllus and use of its extract as antibrowning agent.MolNutr Food Res,2008, Vol. 52, pp. 1530-8.
  11. Kuang Hsu, C., Tien Chang, C., Yi Lub, H., Chin Chung, Y., Inhibitory effects of the water extracts of Lavendulasp. On mushroom tyrosinase activity. Food Chemistry, 2007, Vol. 105,pp.1099-105.
  12. Karioti, A., Protopappa, A., Megoulas, N., Skaltsa, H., Identification of tyrosinase inhibitors from Marrubiumvelutinum andMarrubiumcylleneum. BioorgMedicinal Chem,2007, Vol.15,pp. 2708-14. 
Journal of Applied Biotechnology Reports
Volume 1, Issue 2
June 2014
Pages 63-66

Files

History

  • Receive Date: 25 March 2014
  • Revise Date: 10 May 2014
  • Accept Date: 10 May 2014
  • First Publish Date: 01 June 2014

Share

How to cite

Statistics