Document Type : Original Article
Department of Biology, School of Science and Agriculture, Payame Noor University of Tehran, Tehran, Iran
Department of Medicinal Chemistry, School of Pharmacy, Ahwaz Jundishapur University of Medical Sciences, Ahwaz, Iran
Department of Pharmacognosy, School of Pharmacy, Ahwaz Jundishapur University of Medical Sciences, Ahwaz, Iran
Melanin pigment plays a critical role in camouflage and protection against harmful effects of solar radiation. Melanogenesis is under complex regulatory control by multiple agents. Tyrosinase is a multifunctional, which catalyzes the first two steps in mammalian melanogenesis. In this study, inhibitory effects of Astragalus fasciculifolius and Astragalus gypsicolus on diphenolase activity of mushroom tyrosinase were evaluated. .Ethanol 80% extracts were prepared for screening tests. The IC50, Ki, Km and Vm values of Astragalus fasciculifolius and Astragalus gypsicolus were measured then compared them. We found that Both extracts show mixed type inhibition on mushroom tyrosinase when L-DOPA was used as a substrate.