Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 4 3 2017 09 01 Skin Substitutes; an Updated Review of Products from Year 1980 to 2017 615 623 68655 EN Fariba Mohebichamkhorami Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Akram Alizadeh Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran 0000-0002-9956-4556 Journal Article 2017 04 06 Skin substitutes help skin repair and regeneration, and restore the efficient properties of skin in the time of acute burn injuries or other chronic skin lesions. They can act as permanent skin replacements or temporary wound covers, depending on their composition and, design. Recent studies have overcome some obstacles, but till today no ideal skin substitute has been developed. The aim of this study is to introduce some commercially available and under development products and also to provide information about these substitutes and their limitations in order to use native-like skin substitute design and production. Currently, the accessible skin substitutes have several limitations such as infection risk, reduced vascularization and lack of integration to host tissue. The absence of various cells which are responsible for temperature control and insulation, pigmentation, immune regulation and nerve supply is among the mentioned limitations. Further researches will be required to resolve different issues and suggest practical solutions toward a true skin substitute with excellent engraftment and durable viability. In addition, availability and awareness of these skin substitutes in developing countries is not adequate in spite of the number of cases requiring this kind of treatment, therefore, it is needed to develop indigenous economical technology to promote available treatments in hopes of achieving substitutes with higher quality and reasonable cost available to a greater percentage of patients.

https://www.biotechrep.ir/article_68655_eac7f5b64e43016561d22e53bb5359f0.pdf

Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 4 3 2017 09 01 Biologically Modified Titanium Substrates for Improved Surface Bioactivity 625 631 68656 EN Mahsa Gheysour Stem cell and regenerative medicine group, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran Shahab Faghihi Stem cell and regenerative medicine group, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran Journal Article 2017 07 25 Post-surgery infections and not effective integration represent a serious issue in the Titanium (Ti) based implants function for a long term stability. To reduce such issue various surface functionalization method including surface coating has been explored. Here, we successfully coated Ti substrates with Graphene Oxide (GO), Chitosan (Cs), and nanocomposite of GO and Cs (GO/Cs) via spin coated method to evaluate the osteogenic properties of each coatings. Uncoated Ti substrates were used as control. Scanning electron microscopy was used to investigate the coating morphology. Surface roughness measurements were achieved from atomic force microscopy. To measure surface wettability, contact angels method was performed. Ti substrates coated with Cs (TiCs) and Cs/GO (TiCs/GO) showed the highest surface wettability compared to Ti substrates coated with GO (TiGO) and the control. The highest surface roughness was also observed in TiCs/GO. To test cellular attachment and proliferation the samples were exposed to human osteoblast-like MG63 cells after 2 hours, 4 hours, 6 hours, 1 day, 3 days, and 1 week. MTT [3-4,5 dimethylthiazol-2yl (2,5diphenyl-2H-tetrazoliumbromide)] assay was performed to measure the percentage of cellular attachment and proliferation for each coatings. Cell adhesion and cell proliferation was most improved in TiCs followed by TiCs/GO. Corrosion resistance of the coatings was investigated using potentiodynamic polarization test in simulated body fluid. The result indicated that the nanocomposite coating could provide effective protection of Ti substrates from corrosion.

https://www.biotechrep.ir/article_68656_55f7f89113018d3dffc8ae022b870e13.pdf

Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 4 3 2017 09 01 Utilizing Cell-SELEX, as a Promising Strategy to Isolate ssDNA Aptamer Probes for Detection of Staphylococcus aureus 633 638 68665 EN Mitra Yazdi Yahyaabadi Applied Biotechnology Research Center, Baqiyatallah University of Medical Science, Tehran, Iran Ghambar Sultan Dorraj Applied Biotechnology Research Center, Baqiyatallah University of Medical Science, Tehran, Iran Mohammad Heiat Applied Biotechnology Research Center, Baqiyatallah University of Medical Science, Tehran, Iran Ali Mohammad Latifi 0000-0002-8952-5174 Journal Article 2017 06 06 Staphylococcus aureus is one of the most important pathogens in hospital acquired infections. Annually, many people are infected with S. aureus in hospitals. Rapid detection of this bacterium is extremely helpful in preventing and managing this bacterium mediated diseases. Aptamers are powerful probes, which can be used as a target explorer in a wide range of diagnostic systems. To isolate a specific aptamer against S. aureus, a library of single-stranded DNA molecules was designed, and enriched through Cell-SELEX procedure. In the Cell-SELEX, the DNA library was exposed to the S. aureus bacterium in 8 reiterative quadruple rounds including: binding, separation, elution and amplification. After 8 rounds, the PCR product was cloned and sequenced. Cloned aptameric sequences were evaluated through enzyme-linked oligonucleotide assay (ELONA), and a sequence with the best outcomes was selected as ideal aptamer. Eight rounds of Cell-SELEX procedure led to isolation of a specific ssDNA aptamer against S. aureus and named as “STAPT” (conflation of STAphylococcus and APTamer). Using ELONA technique, the detection limit of this aptamer was determined as 4 × 103 CFU/ml. The aptamer “STAPT” showed the promising and potent abilities and features to be utilized as a bio-detection element likely in advanced detection systems. Although more extended researches are needed for this purpose.

https://www.biotechrep.ir/article_68665_d6a0775887032913b0cd9a82c4ced3e3.pdf

Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 4 3 2017 09 01 Assessment of Changes in Expression of the 5-HT2A and GABAA Receptor Genes in Peripheral Blood Mononuclear Cells of Gas Station Workers, Tehran, Iran 639 643 68667 EN Sheyda Bahiraii Department of Molecular Biology, University of Vienna, Vienna, Austria Ghasem Ahangari Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran Abbas Mirshafiey Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mehdi Razazian Department of Molecular Biology, University of Vienna, Vienna, Austria Farkhondeh Mohammadi Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Journal Article 2017 08 08 Gasoline containing different chemical compounds especially aromatic compounds such as benzene, xylene, toluene and ethylbenzene which are the most dangerous compounds. Gas station workers have the highest potential for exposure to gasoline evaporation. Many studies have investigated the effect of gasoline vapors and cause of death in people working in gas stations. According to reports, the cause of death of these people is mainly due to cardiovascular disease, lung cancer, leukemia and other cancers in the world. With regard to the harmful effects of gasoline vapors on health and the importance of the relationship between the immune system and nervous, in this study we investigated alternations in gene expression pattern of the serotonin 5-Hydroxytryptamine 2A (5-HT2A) receptor and type A gammaaminobutyric acid (GABAA) receptor in peripheral blood mononuclear cells (PBMCs) of people working in gas stations, Tehran. For this purpose, The PBMCs were separated from whole blood of 30 gas station workers and 30 normal by Ficoll-hypaque. The total RNA was extracted and the cDNA was synthesized. This process was followed by Real-time PCR using specific primers for 5-HT2A and GABAA receptor mRNAs. Results have shown a significant increase (P = 0.001) in relative gene expression of 5-HT2A receptor whereas relative expression of GABAA receptor was significantly decreased (P = 0.004) in PBMC compared with normal control peoples. According to the role of the serotonin in asthma and due to the importance of the activity of this neurotransmitter through 5HT2A receptor which has a significant increase in patients with allergic asthma it can be concluded that workers in the gas station may be more susceptible to developing allergic asthma. On the other hand, low expression of GABAA receptor is associated with mental health problems such as depression, so it can also be concluded that people working at the gas station are more exposed to these health problems in comparison to normal people.

https://www.biotechrep.ir/article_68667_0db92c07aea22acde7d7e62eb5e734db.pdf

Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 4 3 2017 09 01 Cell Wall Extract from Piriformospora indica Enhances Valerenic Acid Content in Valerian Roots via Modulating Sesquiterepene Synthase Genes 645 650 68964 EN Mansour Ghesmati Department of Agricultural Biotechnology, Ramin Agriculture and Natural Resources University of Khouzestan, Iran Hengameh Taheri Department of Agricultural Biotechnology, Ramin Agriculture and Natural Resources University of Khouzestan, Iran Ali Akbar Meratan Department of Biological Sciences, Institute in Advanced Studies in Basic Sciences (IASBS), Zanjan, Iran Payam Pour Mohamadi Departmentof Agronomy and Plant Breeding, Ramin Agriculture & Natural Resources University of Khouzestan, Iran Journal Article 2017 07 15 https://www.biotechrep.ir/article_68964_1d851a9861cc3139707b4a2c4a5de601.pdf

Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 4 3 2017 09 01 Determination of Sensitivity Level of Hypocotyl Explants to Paromomycin Antibiotic and Optimization of Rooting in Transgenic Medicinal Plant Papaver somniferum 651 655 68965 EN Parvin Norouzi Faculty of Agriculture, Lorestan University, Khorramabad, Iran Ahmad Ismaili Faculty of Agriculture, Lorestan University, Khorramabad, Iran Farhad Nazarian Firouz-Abadi Faculty of Agriculture, Lorestan University, Khorramabad, Iran Ali Mohammad Latifi Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran Journal Article 2017 04 05 Poppy (Papaver somniferum) is the most important commercial source of analgesic drugs and of compounds related to them. Because of the excellent medicinal prop- erties of poppy, finding genotypes with changed alkaloid content through breeding or by producing transgenic plants in tissue culture has attracted great interest. The present research was conducted to optimize rooting in somatic embryos of trans- genic poppy plants. Sensitivity of hypocotyl explants to the antibiotic paromomycin was first determined in kill-curve experiments. Transformation of the hypocotyl explants was carried out using the GV3101 strain of Agrobacterium tumefaciens having the vector with the NPT II gene. The explants were then transferred to MS- based medium containing the 2, 4-D plant growth regulator (PGR) at 1 mg/L, 10 mM MES, and paromomycinat 15 mg/L. After the somatic embryos were pro- duced, a factorial experiment was conducted in the rooting stage with two factors including medium (MS and 1/2 MS) and PGR (500 μM IBA PGR and no PGR). Also, confirmation of derived transgenic plantlets was confirmed by PCR tech- nique. Results of ANOVA and comparison of the means indicated the highest root- ing percentage happened in the 1/2 MS medium containing the IBA. https://www.biotechrep.ir/article_68965_04fe3a85bc471f3a3f0211283a3b0e4a.pdf

Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 4 3 2017 09 01 The Genetic and Phytochemical Diversity of Four Populations of Lemongrass (Cymbopogon olivieri) from Southeast Iran 657 660 68966 EN Zeynab Kahnouji Department of Biotechnology, Faculty of Agriculture, Shahid Bahonar University of Kerman, Iran Gholam-Reza Sharifi-Sirchi Department of Agriculture, Faculty of Agriculture and Natural Sources, University of Hormozgan, Bandar Abbas, Iran Hossein Ali Sassan Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Iran Journal Article 2017 06 03 Lemongrass (Cymbopogon olivieri) is highly cross-pollinated and fragrant herb plant. Because of so worthy essential oil components, it is widely used in cosmetics, food, and medicine industry. The C. olivieri leaves of 4 populations were sampled from South and Southeast Iran. Random amplified polymorphic DNA (RAPD) markers used to assess the population structure and genetic variation. In total, 32 polymorphic bands amplified from 11 effective chosen RAPD markers. Cluster analysis using the UPGMA method divided the populations into 2 main groups. A high cophenetic correlation coefficient (r = 0.90) was obtained. The pale yellow essential oils were used for GC-MS analysis. Pipertone, carrene, elemol, limonene, benzene, α-pinene, linalool, azulene, and calarene were the components with the highest amount found in this study. The high genetic and phytochemical diversity among studied C. olivieri population was observed which could be applied in following breeding and gene bank conservation programs. https://www.biotechrep.ir/article_68966_3ccf50b9d73ea11f758bd030e5ac593f.pdf

Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 4 3 2017 09 01 Investigation on Crystallinity Behavior of the Polylactic Acid and Poly-3-hydroxybutyrate Bio-based Polymers in the Presence of the Pyromellitic Anhydride 661 668 68967 EN Tayebeh Pourjafar Devin Department of Chemistry, Amirkabir University of Technology, Tehran, Iran Zahed Ahmadi Department of Chemistry, Amirkabir University of Technology, Tehran, Iran Faramarz Afshari Taromi Department of Polymer Engineering and Color Technology, Amirkabir University of Technology, Tehran, Iran Journal Article 2017 06 02 Bio-based polymers have attracted significant attentions because of their renewable nature which can be a substitute of petroleum-based polymers; moreover, such polymers do not pollute the environment due to their degradable nature. In this research,the poly lactic acid (PLA) and poly-3-hydroxybutyrate (PHB) blend, as biodegradable polymers, behavior was investigated. Different ratios of PLA/PHB blend were prepared using solution casting method in the presence of the pyromel- litic anhydride (PMDA) as acompatibilizer. DSC results revealed that the PMDA presence caused to separation of α-helix crystals and β-plate in two polymers re- sulting in phase separation via altering the crystallinity rate. PMDA can control the crystallization phenomenon which affected the crystallization rate, percentage and crystal size. Low molecular interaction was observed using the XRD results of pure and blended samples. SEM images of samples with higher content of PLA illu- strated the homogeneous dispersion of PHB. Ring opening reaction of PMDA re- sulted in increasing the acidic number of samples which indicated the weak interac- tion with active group of polymers. It was observed no shit in IR peaks which indi- cated the incompatibility of these two polymers. Results shed light on the PMDA behavior in PLA/PHB blend which PMDA in spite of controlling the crystallinity behavior of the blend, cannot improve the compatibility and mixing. https://www.biotechrep.ir/article_68967_cd3b315eabf4e2035b65bb357a8eaf8d.pdf

Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 4 3 2017 09 01 Electrochemical Behavior of Adult and Fetal Hemoglobin at Gold-coated Magnetic Iron Oxide Nanoparticle 669 674 68968 EN Khadijeh Eskandari Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Hedayatollah Ghourchian Laboratory of Microanalysis, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran Aghdas Banaei Research Institute of Applied Science, Academic Center of Education, Culture and Research (ACECR), Shahid Beheshti University, Tehran, Iran Journal Article 2017 05 24 A feasible and fast method for Adult Hemoglobin (A-Hb) and Fetal Hemoglobin (F-Hb) study was developed by immobilization of Hb on gold-coated magnetic iron oxide nanoparticles (GMNPs).The prepared GMNPs composite nanoparticles with 60 nm diameter were used as a carrier for the immobilization of Hb. The A-Hb and F-Hb were physically attached to the GMNPs nanoparticles. The direct electroche- mistry of F-Hb and A-Hb showed a quasi-reversible cyclic voltammogram corres- ponding to the Heme group with a formal potential of 314 and -334 mV in 0.1M PBS (pH 6.2), respectively. The apparent charge transfer rate constant (ks) and transfer coefficient (α) for electron transfer between the electrode surface and pro- tein were calculated as 0.29/s and 0.1 for F-Hb and 0.21/s and 0.47 for A-Hb. The linear concentration rangesare17.3–225 and 7.4-53 mM for F-H band A-Hb biosensors for H O detection. The lifetime of biosensor is more than 2 weeks.

https://www.biotechrep.ir/article_68968_8d0d2b57bdc0fa64bc3348ffcfd5d159.pdf